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Supramolecular Coassembly Activated Dual-Excitation Fluorescent Sensing Platform for Precise Detection of Aflatoxin B.
Anal Chem
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
The development of a sensory signal amplification approach is very crucial for rapid and precise detection of aflatoxin B (AFB). However, such approaches remain scarce due to the weak interactions between AFB and the sensing probes. Herein, the first example of a dual-excitation fluorescent platform for antibody-free AFB detection is reported, which is assembled by an ordered π-π stack of cationic perylene derivative (PTHA) and tris(2,2'-bipyridine)ruthenium(II) [Ru(bpy)].
View Article and Find Full Text PDFBioengineering chitosan-antibody/fluorescent quantum dot nanoconjugates for targeted immunotheranostics of non-hodgkin B-cell lymphomas.
Int J Biol Macromol
January 2025
Center of Nanoscience, Nanotechnology, and Innovation - CeNano2I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, UFMG, Brazil. Electronic address:
B-cell non-Hodgkin lymphoma (NHL) is the most common hematologic malignancy, capable of invading the brain, meninges, and nerve roots of the brain and spine, leading to high lethality. Herein, we designed and developed novel nanostructures for the first time by biofunctionalizing chitosan with two specific antibodies (i.e.
View Article and Find Full Text PDFRobust visualization of membrane protein by aptamer mediated proximity ligation assay and Förster resonance energy transfer.
Colloids Surf B Biointerfaces
December 2024
Key Laboratory of Marine Drug, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266237, China. Electronic address:
In situ cell imaging plays a crucial role in studying physiological and pathological processes of cells. Proximity ligation assay (PLA) and rolling circle amplification (RCA) are commonly used to study the abundance and interactions of biological macromolecules. The most frequently applied strategy to visualize the RCA products is with single-fluorophore probe, however, cellular auto-fluorescence and unbound fluorescent probes could interfere with RCA products, leading to non-specific signals.
View Article and Find Full Text PDFOrthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET.
Sci Rep
January 2025
Safety Innovation and PROTAC Safety, Clinical Pharmacology & Safety Sciences, R&D, AstraZeneca, Cambridge, CB2 0SL, UK.
Dysregulation of integral membrane proteins (IMPs) has been linked to a myriad of diseases, making these proteins an attractive target in drug research. Whilst PROTAC technology has had a significant impact in scientific research, its application to IMPs is still limited. Limitations of the traditional approach of immunoblotting in PROTAC research include the low throughput compared to other methods, as well as a lack of spatial information for the target.
View Article and Find Full Text PDFThe spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer.
Nat Commun
January 2025
MultiplexDX, s.r.o., Comenius University Science Park, Bratislava, Slovakia.
Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, and explicitly quantifies intratumoral heterogeneity.
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