Background: Phosphopantetheinyl transferase (PPTase) can change the acyl-carrier protein (ACP) from an inactive apo-ACP to an active holo-ACP that plays a key role in fatty acids biosynthesis. Currently, the PPTase has been proved to be involved in the biosynthesis of polyunsaturated fatty acids (PUFAs) via a polyketide synthase (PKS) pathway in Thraustochytrids, while its characteristics are not clarified.
Results: Here, the heterologous PPTase gene () from bacteria was first co-expressed with the PKS system (-) from Thraustochytrid . Then, a new endogenous PPTase () in was identified by homologous alignment and its function was verified in . Moreover, the endogenous was then overexpressed in , and results showed that the production and proportion of PUFAs, especially docosahexaenoic acid (DHA), in the transformant SD116::PPT_A were increased by 35.5% and 17.6%, respectively. Finally, higher DHA and PUFA proportion (53.9% and 64.5% of TFA, respectively) were obtained in SD116::PPT_A using a cerulenin feeding strategy.
Conclusions: This study has illustrated a PUFAs-synthase-specific PPTase in PKS system and provided a new strategy to improve the PUFA production in Thraustochytrids.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457351 | PMC |
| http://dx.doi.org/10.1186/s13068-020-01793-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Commentary on "Cross-sectional associations between dietary intake of polyunsaturated fatty acids, physical function, and sarcopenia in community-dwelling older adults".
J Nutr Health Aging
January 2025
Gazi University, School of Medicine, Department of Internal Medicine, Division of Geriatrics, Ankara, Turkey.
is a putative producer of polyunsaturated fatty acids in the gut soil of the composting earthworm .
Appl Environ Microbiol
January 2025
Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, University of Vienna, Vienna, Austria.
Polyunsaturated fatty acids (PUFAs) play a crucial role in aiding bacteria to adapt to extreme and stressful environments. While there is a well-established understanding of their production, accrual, and transfer within marine ecosystems, knowledge about terrestrial environments remains limited. Investigation of the intestinal microbiome of earthworms has illuminated the presence of PUFAs presumably of microbial origin, which contrasts with the surrounding soil.
View Article and Find Full Text PDFProductive performance, rumen parameters, carcass quality, antioxidant profile and methane emission in lambs supplemented with triticale hay.
Vet Anim Sci
March 2025
Animal Science Research Department, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
This study aims to measure the effects of different dietary concentrations of triticale hay (TH) on productive performance, carcass characteristics, microbial protein synthesis (MPS), ruminal and blood variables, and antioxidant power in 40 fattening male Gray Shirazi lambs (BW of 33.2 ± 1.1 kg) over 81 days in a completely randomized design (10 animals/diet).
View Article and Find Full Text PDFALOX15 Aggravates Metabolic Dysfunction-Associated Steatotic Liver Disease in Mice with Type 2 Diabetes via Activating the PPARγ/CD36 Axis.
Antioxid Redox Signal
January 2025
Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent hepatic disorder worldwide. Arachidonic acid 15-lipoxygenase (ALOX15), an enzyme catalyzing the peroxidation of polyunsaturated fatty acids, plays a crucial role in various diseases. Here, we sought to investigate the involvement of ALOX15 in MASLD.
View Article and Find Full Text PDFPlasma levels of anti phosphocholine IgM antibodies are negatively correlated with bone mineral density in humans.
Sci Rep
January 2025
Division of Endocrinology and Metabolism and Center for Musculoskeletal Disease Research, University of Arkansas for Medical Sciences, 4301 W. Markham, #587, Little Rock, AR, 72205, USA.
Phosphatidylcholine is a ubiquitous phospholipid. It contains a phosphocholine (PC) headgroup and polyunsaturated fatty acids that, when oxidized, form reactive oxidized phospholipids (PC-OxPLs). PC-OxPLs are pathogenic in multiple diseases and neutralized by anti-PC IgM antibodies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!