Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells' metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 - myoblasts, and B16-F10 - melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10895-020-02611-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibacterial properties and biological activity of 3D-printed titanium alloy implants with a near-infrared photoresponsive surface.
Int J Implant Dent
January 2025
School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, 310053, People's Republic of China.
Purpose: SLM 3D printing technology is one of the most widely used implant-making technologies. However, the surfaces of the implants are relatively rough, and bacteria can easily adhere to them; increasing the risk of postoperative infection. Therefore, we prepared a near-infrared photoresponsive nano-TiO coating on the surface of an SLM 3D-printed titanium alloy sheet (Ti6Al4V) via a hydrothermal method to evaluate its antibacterial properties and biocompatibility.
View Article and Find Full Text PDFFatal and sp. co-infestation in wolves () at the Białowieża National Park, Poland - is it a consequence of climate change?
J Vet Res
December 2024
Department of Virology, National Veterinary Research Institute, 24-100 Puławy, Poland.
Introduction: In winter 2021/2022, a wolf population in the primeval Białowieża Forest in Poland was struck by an outbreak of severe mange caused by mixed infestations of and mites. We present an epidemiological analysis of this mange which caused significant morbidity and mortality.
Material And Methods: Ten sites known for wolf activity were monitored by camera trapping.
Timing of Delivery of Low-Risk Persons and the Risk of Attention-Deficit Hyperactivity Disorder in Offspring: Sweden and British Columbia, Canada.
Paediatr Perinat Epidemiol
January 2025
Clinical Epidemiology Division, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Background: An evidence gap exists concerning the timing of delivery at 37-42 weeks and the risk of attention-deficit hyperactivity disorder (ADHD) in offspring.
Objective: To determine the association between timing of delivery in low-risk pregnancies at term (37-42 weeks) gestations and ADHD in offspring.
Methods: This population-based cohort study comprised 1,424,453 singletons in Sweden and 403,765 in British Columbia (BC), Canada, live-born at 37-42 completed weeks to low-risk pregnant women between 2000 and 2018.
Sex-specific differences in mortality and neurocardiac interactions in the Kv1.1 knockout mouse model of sudden unexpected death in epilepsy (SUDEP).
J Physiol
January 2025
Department of Biological Sciences, Southern Methodist University, Dallas, TX, USA.
Sudden unexpected death in epilepsy (SUDEP) is a devastating complication of epilepsy with possible sex-specific risk factors, although the exact relationship between sex and SUDEP remains unclear. To investigate this, we studied Kcna1 knockout (Kcna1) mice, which lack voltage-gated Kv1.1 channel subunits and are widely used as a SUDEP model that mirrors key features in humans.
View Article and Find Full Text PDFInnovative Methodology for Antimicrobial Susceptibility Determination in Biofilms.
Microorganisms
December 2024
Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59718, USA.
spp. are facultative pathogens that contribute to the pathogenesis of multiple bovine diseases, including the bovine respiratory disease complex, and have been shown to form biofilms. Biofilm formation is associated with increased antibiotic resistance in many organisms, but accurate determination of antimicrobial susceptibility in biofilms is challenging.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!