AI Article Synopsis
- The text discusses the emerging technology of Magnetic Torque Stimulation (MTS) for regulating cell signaling through physical stimulation in biomedicine.
- MTS uses engineered magnetic particles and an external rotating magnetic field to apply forces on heart cells, specifically cardiomyocytes and cardiac fibroblasts, promoting their maturation and hypertrophy.
- Findings indicate that MTS enhances important biological responses under low-oxygen conditions, suggesting its potential for remote and noninvasive applications in various cell studies.
Article Abstract
Unlabelled: Regulation of cell signaling through physical stimulation is an emerging topic in biomedicine.
Background: While recent advances in biophysical technologies show capabilities for spatiotemporal stimulation, interfacing those tools with biological systems for intact signal transfer and noncontact stimulation remains challenging. Here, we describe the use of a magnetic torque stimulation (MTS) system combined with engineered magnetic particles to apply forces on the surface of individual cells. MTS utilizes an externally rotating magnetic field to induce a spin on magnetic particles and generate torsional force to stimulate mechanotransduction pathways in two types of human heart cells-cardiomyocytes and cardiac fibroblasts.
Methods: The MTS system operates in a noncontact mode with two magnets separated (60 mm) from each other and generates a torque of up to 15 pN µm across the entire area of a 35-mm cell culture dish. The MTS system can mechanically stimulate both types of human heart cells, inducing maturation and hypertrophy.
Results: Our findings show that application of the MTS system under hypoxic conditions induces not only nuclear localization of mechanoresponsive YAP proteins in human heart cells but also overexpression of hypertrophy markers, including β-myosin heavy chain (βMHC), cardiotrophin-1 (CT-1), microRNA-21 (miR-21), and transforming growth factor beta-1 (TGFβ-1).
Conclusions: These results have important implications for the applicability of the MTS system to diverse in vitro studies that require remote and noninvasive mechanical regulation.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557977 | PMC |
| http://dx.doi.org/10.3390/nano10091684 | DOI Listing |
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