Purpose: Liver cancer stem cells are associated with tumor progression, metastasis, and resistance to chemotherapy. Therefore, it is important to understand the proteins that support the tumor microenvironment. The suppression of ZEB2 results from inactivation of the Wnt/β catenin pathway. Like RBM38, it suppresses tumor outgrowth and helps increase the survival of cancer patients. However, no studies have examined the direct roles of ZEB2 and RBM38 in the tumor microenvironment.
Methods: We developed an early/advanced stage liver cancer mouse model using CD133+ cell injection that mimics liver cancer in all ways. Histology, Western blotting, and immunohistochemistry analyses were used to examine cancer progression.
Results: Histologically, the early liver cancer showed microfoci structures; the advanced cancer showed distinct morphological changes with enlarged nucleoli and cell clumping. Immunohistochemical and Western blotting analyses of CD133 and ZEB2 proteins showed similar upregulated expression as the tumor progressed. However, RBM38 expression increased dramatically in early liver cancer but was downregulated in advanced liver cancer.
Conclusions: ZEB2 favors a tumor microenvironment that supports liver cancer stem cell proliferation, while RBM38 expression negatively affects the tumor microenvironment and restricts liver cancer stem cell proliferation.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Cuproptosis Cell Death Molecular Events and Pathways to Liver Disease.
J Inflamm Res
January 2025
Department of Infectious Disease, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, People's Republic of China.
Chronic liver disease ranks as the 11th leading cause of death worldwide, while hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related mortality, representing a substantial risk to public health. Over the past few decades, the global landscape of chronic liver diseases, including hepatitis, metabolic dysfunction-associated steatotic liver disease (MASLD), liver fibrosis, and HCC, has undergone substantial changes. Copper, a vital trace element for human health, is predominantly regulated by the liver.
View Article and Find Full Text PDFPrognosis prediction of α-FAtE score for locoregional immunotherapy in hepatocellular carcinoma.
Front Immunol
January 2025
Department of Liver Surgery, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
Purpose: The α-FAtE score, composed of alpha-fetoprotein, alkaline phosphatase, and eosinophil levels, has been reported as a predictor of prognosis in hepatocellular carcinoma (HCC) patients treated with atezolizumab plus bevacizumab. This study aimed to investigate the predictive ability of α-FAtE score for the efficacy and safety of locoregional immunotherapy as the treatment of HCC patients.
Methods And Patients: We conducted a retrospective study of 446 HCC patients at Sun Yat-sen University Cancer Center from January 1 2019 to January 1 2023.
Cohort study on the treatment of mutant metastatic colorectal cancer with integrated Chinese and western medicine.
World J Clin Oncol
January 2025
Department of Oncology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China.
Background: Patients with mutant metastatic colorectal cancer (mCRC) have a low incidence rate, poor biological activity, suboptimal response to conventional treatments, and a poor prognosis. In the previous cohort study on mCRC conducted by our team, it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival (OS) of patients with colorectal cancer. Therefore, we further explored the survival benefits in the population with mutant mCRC.
View Article and Find Full Text PDFDopamine receptors and organ fibrosis.
Biochem Biophys Rep
March 2025
Department of Clinical Nutrition, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 400030, China.
Organ fibrosis, considered as a major global health concern, is a pathological condition often occurring after tissue injury in various organs. The pathogenesis of fibrosis involves multiple phases and multiple cell types. Dopamine is involved in various life activities by activating five receptors (D1, D2, D3, D4, D5).
View Article and Find Full Text PDFClinical efficacy and safety evaluation of traditional Chinese medicine for nourishing yin and Replenishing qi in combination with PD-1/PD-L1 inhibitors in the treatment of NSCLC patients: a meta-analysis.
Toxicol Res (Camb)
February 2025
Respiratory Medicine, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, 71 Baoshan North Road, Yunyan District, Guiyang Guizhou 550001, China.
To examine the therapeutic effectiveness and safety of traditional Chinese medicine in conjunction with PD-1/PD-L1 inhibitors for nourishing yin and replenishing qi in patients with non-small cell lung cancer. A systematic search was conducted across seven electronic databases, namely PubMed, Cochrane Library, Excerpt Medica Database, Web of Science, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, and Wan-fang Database, to identify eligible studies from 2,000 to 2,023. This study includes a total of 14 randomized controlled clinical trials, with 514 patients in the TCM combo therapy group and 506 patients in the control group.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!