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Isolation of Cancer-Derived Exosomes Using a Variety of Magnetic Nanostructures: From FeO Nanoparticles to Ni Nanowires. | LitMetric

AI Article Synopsis

  • Isolating tumor-derived exosomes (TEX) is crucial for early tumor diagnosis and prognosis, but current methods are complex and costly.
  • The proposed faster and cheaper solution involves using magnetic nanostructures (Ni nanowires, FeO nanorods, and cube-octahedral magnetosomes) for TEX isolation.
  • Results showed that Ni magnetic nanowires provided better yield and purity of TEX compared to other nanostructures, nearly matching traditional methods.

Article Abstract

Isolating and analyzing tumor-derived exosomes (TEX) can provide important information about the state of a tumor, facilitating early diagnosis and prognosis. Since current isolation methods are mostly laborious and expensive, we propose herein a fast and cost-effective method based on a magnetic nanoplatform to isolate TEX. In this work, we have tested our method using three magnetic nanostructures: (i) Ni magnetic nanowires (MNWs) (1500 × 40 nm), (ii) FeO nanorods (NRs) (41 × 7 nm), and (iii) FeO cube-octahedral magnetosomes (MGs) (45 nm) obtained from magnetotactic bacteria. The magnetic response of these nanostructures has been characterized, and we have followed their internalization inside canine osteosarcoma OSCA-8 cells. An overall depiction has been obtained using a combination of Fluorescence and Scanning Electron Microscopies. In addition, Transmission Electron Microscopy images have shown that the nanostructures, with different signs of degradation, ended up being incorporated in endosomal compartments inside the cells. Small intra-endosomal vesicles that could be precursors for TEX have also been identified. Finally, TEX have been isolated using our magnetic isolation method and analyzed with a Nanoparticle tracking analyzer (NanoSight). We observed that the amount and purity of TEX isolated magnetically with MNWs was higher than with NRs and MGs, and they were close to the results obtained using conventional non-magnetic isolation methods.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558559PMC
http://dx.doi.org/10.3390/nano10091662DOI Listing

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