Grapevine downy mildew caused by is one of the most important diseases in vineyards. Oospores that overwinter in the leaf litter above the soil are the sole source of inoculum for primary infections of ; in addition to triggering the first infections in the season, the oospores in leaf litter also contribute to disease development during the season. In the current study, a quantitative polymerase chain reaction (qPCR) method that was previously developed to detect DNA in fresh grapevine leaves was assessed for its ability to quantify oospores in diseased, senescent grapevine leaves. The qPCR assay was specific to and sensitive to decreasing amounts of both genomic DNA and numbers of oospores used to generate qPCR standard curves. When the qPCR assay was compared to microscope counts of oospores in leaves with different levels of infestation, a strong linear relationship (R = 0.70) was obtained between the numbers of oospores per gram of leaves as determined by qPCR vs. microscopic observation. Unlike microscopic observation, the qPCR assay was able to detect significant differences between leaf samples with a low level of oospore infestation (25% infested leaves and 75% non-infested leaves) vs. samples without infestation, and this ability was not influenced by the weight of the leaf sample. The results indicate that the qPCR method is sensitive and provides reliable estimates of the number of oospores in grapevine leaves. Additional research is needed to determine whether the qPCR method is useful for quantifying oospores in grapevine leaf litter.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426466 | PMC |
| http://dx.doi.org/10.3389/fpls.2020.01202 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Status of Wild Grapevine ( L. subsp. (C.C. Gmel.) Hegi) Populations in Georgia (South Caucasus).
Plants (Basel)
January 2025
National Wine Agency of Georgia, Tbilisi 0159, Georgia.
Repeated expeditions across various regions of Georgia in the early 2000s led to the identification of 434 wild grapevine individuals ( L. subsp. (C.
View Article and Find Full Text PDFCombining a Standardized Growth Class Assessment, UAV Sensor Data, GIS Processing, and Machine Learning Classification to Derive a Correlation with the Vigour and Canopy Volume of Grapevines.
Sensors (Basel)
January 2025
Department of Environmental Remote Sensing and Geoinformatics, Trier University, Universitätsring 15, 54296 Trier, Germany.
Assessing vines' vigour is essential for vineyard management and automatization of viticulture machines, including shaking adjustments of berry harvesters during grape harvest or leaf pruning applications. To address these problems, based on a standardized growth class assessment, labeled ground truth data of precisely located grapevines were predicted with specifically selected Machine Learning (ML) classifiers (Random Forest Classifier (RFC), Support Vector Machines (SVM)), utilizing multispectral UAV (Unmanned Aerial Vehicle) sensor data. The input features for ML model training comprise spectral, structural, and texture feature types generated from multispectral orthomosaics (spectral features), Digital Terrain and Surface Models (DTM/DSM- structural features), and Gray-Level Co-occurrence Matrix (GLCM) calculations (texture features).
View Article and Find Full Text PDFManganese biofortification in grapevine by foliar spraying improves volatile profiles of Cabernet Sauvignon grapes and wine sensory traits.
Food Chem X
January 2025
College of Enology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, Shaanxi, China.
Manganese (Mn) is involved in plant metabolism as an enzyme cofactor. However, the role of Mn in the formation of volatile compounds in grapes has rarely been studied. To address this gap, this study explored the effect of foliar Mn application on the aroma traits of grapes and wine.
View Article and Find Full Text PDFOverexpression of the Vitis amurensis Ca-binding protein gene VamCP1 in Arabidopsis thaliana and grapevine improves cold tolerance.
Physiol Plant
January 2025
College of Enology and Horticulture, Ningxia University/College of Modern Grape and Wine Industry/Ningxia Grape and Wine Research Institute/Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan, P.R. China.
Calcium ions (Ca) are important second messengers and are known to participate in cold signal transduction. In the current study, we characterized a Ca-binding protein gene, VamCP1, from the extremely cold-tolerant grape species Vitis amurensis. VamCP1 expression varied among organs but was highest in leaves following cold treatment, peaking 24 h after treatment onset.
View Article and Find Full Text PDFA high-throughput ResNet CNN approach for automated grapevine leaf hair quantification.
Sci Rep
January 2025
Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, Germany.
The hairiness of the leaves is an essential morphological feature within the genus Vitis that can serve as a physical barrier. A high leaf hair density present on the abaxial surface of the grapevine leaves influences their wettability by repelling forces, thus preventing pathogen attack such as downy mildew and anthracnose. Moreover, leaf hairs as a favorable habitat may considerably affect the abundance of biological control agents.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!