Individual chemical and non-chemical treatments have failed to disinfect alfalfa seeds and sprouts from pathogens thoroughly. This study investigated the disinfection of alfalfa seeds and sprouts using a procedure combining ozone with acidic (pH 3.0) electrolyzed water (AEW). Inoculated alfalfa seeds with a cocktail of 3 strains Salmonella and 3 strains of STEC were treated sequentially with aqueous ozone followed by AEW. Treatment started by immersing the samples into ozonated water (5 mg/L ozone) for 15 or 20 min with persistent oxygen feeding pressurized with 10 psi. The samples then were immersed in 1 L of AEW for 15 min. Salmonella and STEC were significantly (P < 0.05) reduced by 3.6 and 2.9 log CFU/g on seeds respectively, and by 3.1 and 3.0 log CFU/g reduction on sprouts. Significant differences (P < 0.05) were found in the magnitude of the log reduction between Salmonella and STEC on seeds and between seeds and sprouts. Using combined treatments showed no significant changes in the quality, including shelf life, weight, and color in sprouts as compared to controls. The findings suggest that the combination of ozone and AEW is effective in inactivation of Salmonella and STEC on alfalfa seeds and sprouts with no adverse effects on sprouts quality.
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http://dx.doi.org/10.1016/j.foodres.2020.109488 | DOI Listing |
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Inner Mongolia Grassland Station, Huhhot, Inner Mongolia 010020, China. Electronic address:
Owing to the complicated geographical locations and climates, cultivation and selection of forage seeds are challenging. For the first time, we qualitatively distinguished the drought and cold resistance of forage seeds with the time domain and refractive index spectra using terahertz (THz) time-domain spectroscopy. A multilayer structure propagation (MSP) model was developed based on the effective medium and light transport theory to reveal the underlying biological mechanisms of drought and cold resistance of forage seeds.
View Article and Find Full Text PDFHortic Res
January 2025
Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Key Laboratory of Oasis Town and Mountain-basin System Ecology of Xinjiang Production and Construction Corps, College of Life Sciences, Shihezi University, Shihezi 832003, China.
Alfalfa is one of the most economically valuable forage crops in the world. However, molecular cytogenetic studies in alfalfa lag far behind other cash crops and have reached a bottleneck. Here, we developed a novel chromosome identification system by designing 21 oligo probes in specific regions of each chromosome, which can be used as a barcode to simultaneously distinguish all chromosomes in a cell.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Agricultural, Forest and Transport Machinery, University of Life Sciences in Lublin, Głęboka 28, 20-612, Lublin, Poland.
Int J Food Microbiol
January 2025
Department of Food Science and Technology, The Ohio State University, Columbus, OH, USA; Department of Microbiology, The Ohio State University, Columbus, OH, USA. Electronic address:
Shiga toxin-producing Escherichia coli (STEC), a significant cause of foodborne illnesses, is often associated with the consumption of fresh produce, including alfalfa sprouts. This study was executed to determine how quickly STEC grows, adapts, and colonizes alfalfa sprouts during production and storage, and whether the pathogen's virulence and infectious doses are affected by physiological adaptation to sprouts as an environment. A reporter STEC O157:H7 EDL933 strain was developed to track the transcription of eae, a virulence gene involved in colonizing human intestinal enterocytes.
View Article and Find Full Text PDFFood Chem
February 2025
Department of Food Science and Technology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand 248002, India. Electronic address:
Phytoestrogens, derived from plants possesses structural similarity with 17 β-estradiol found in mammals. It is abundantly present in soybean along with red clove, alfalfa as well as other legumes, nuts, vegetables and seeds. It is used as hormone replacement therapy and exhibits both anti-estrogenic and estrogenic properties that linked to therapeutic benefits as well as plays active role in sports nutrition.
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