A-kinase anchoring protein 350 (AKAP350) is a centrosomal/Golgi scaffold protein, critical for the regulation of microtubule dynamics. AKAP350 recruits end-binding protein 1 (EB1) to the centrosome in mitotic cells, ensuring proper spindle orientation in epithelial cells. AKAP350 also interacts with p150, the main component of the dynactin complex. In the present work, we found that AKAP350 localized p150 to the spindle poles, facilitating p150/EB1 interaction at these structures. Our results further showed that the decrease in AKAP350 expression reduced p150 localization at astral microtubules and impaired the elongation of astral microtubules during anaphase. Overall, this study provides mechanistic data on how microtubule regulatory proteins gather to define microtubule dynamics in mitotic cells.
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