AI Article Synopsis
- The organization of the genome within the cell nucleus is crucial for various cellular functions like gene expression and DNA repair.
- A new method has been developed to measure the pairing of DNA locations during homologous recombination at double-strand breaks in yeast cells.
- This technique uses a tagging system to visualize DNA interactions and confirms the repair products, revealing the timing and physical contact between homologous DNA during the repair process.
Article Abstract
The precise organization of the genome inside the cell nucleus is vital to many cell functions including gene expression, cell division, and DNA repair. Here we describe a method to measure pairing of DNA loci during homologous recombination (HR) at a site-specific double-strand break (DSB) in Saccharomyces cerevisiae. This method utilizes a chromosome tagging system in diploid yeast cells to visualize both the DNA at the break site and the homologous DNA that serves as a repair template. DNA repair products are confirmed in parallel by genomic blot. This visualization method provides insight into the physical contact that occurs between homologous loci during HR and correlates physical interaction with the timing of DNA repair.
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| http://dx.doi.org/10.1007/978-1-0716-0644-5_18 | DOI Listing |
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