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Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater. | LitMetric

Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater.

Curr Opin Environ Sci Health

Section of Microbiology, Virology and Biotechnology, Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona, Catalonia, Spain.

Published: October 2020

AI Article Synopsis

Article Abstract

As the novel SARS-CoV-2 was detected in faeces, environmental researchers have been using centrifugal ultrafiltration, polyethylene glycol precipitation and aluminium hydroxide flocculation to describe its presence in wastewater samples. High recoveries (up to 65%) are described with electronegative filtration when using surrogate viruses, but few literature reports recovery efficiencies using accurate quantification of enveloped viruses. Considering that every single virus will have a different behaviour during viral concentration, it is recommended to use an enveloped virus, and if possible, a betacoronaviruses as murine hepatitis virus, as a surrogate. In this review, we show new data from a newly available technology that provides a quick ultrafiltration protocol for SARS-CoV-2. Wastewater surveillance is an efficient system for the evaluation of the relative prevalence of SARS-CoV-2 infections in a community, and there is the need of using reliable concentration methods for an accurate and sensitive quantification of the virus in water.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437508PMC
http://dx.doi.org/10.1016/j.coesh.2020.08.002DOI Listing

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