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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299457 | PMC |
| http://dx.doi.org/10.1007/s10346-020-01456-w | DOI Listing |
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PrimerEvalPy: a tool for in-silico evaluation of primers for targeting the microbiome.
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Background: The selection of primer pairs in sequencing-based research can greatly influence the results, highlighting the need for a tool capable of analysing their performance in-silico prior to the sequencing process. We therefore propose PrimerEvalPy, a Python-based package designed to test the performance of any primer or primer pair against any sequencing database. The package calculates a coverage metric and returns the amplicon sequences found, along with information such as their average start and end positions.
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