AI Article Synopsis
- This chapter outlines a high-throughput sequencing method tailored for Coronaviruses and similar positive strand viruses, aiming to generate a comprehensive dataset.
- The process involves working with infectious bronchitis virus, which is cultivated in embryonated eggs and collected from the allantoic fluid.
- The protocol consists of three key steps: enriching the allantoic fluid through ultracentrifugation, extracting total RNA, and preparing sequencing libraries from that RNA for DNA sequencing.
Article Abstract
This chapter reports the high-throughput sequencing protocol for sequencing Coronaviruses and other positive strand viruses to produce a dataset of significant depth of coverage. The protocol describes sequencing of infectious bronchitis virus propagated in embryonated eggs and harvested in the allantoic fluid. The protocol is composed of three main steps-enrichment of the allantoic fluid using ultracentrifugation, extraction of total RNA from allantoic fluid, and library preparation from total RNA to DNA sequencing libraries. The workflow will be suitable for all coronaviruses using high-throughput sequencing platforms.
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| http://dx.doi.org/10.1007/978-1-0716-0900-2_5 | DOI Listing |
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