The tail of tadpole is an excellent model for appendage regeneration studies. We analyzed the distribution pattern of the transcription factor Xvent-2 mRNA and protein in the beginning of the regeneration of tadpole tail stumps after amputation. We revealed the emergence of Xvent-2 mRNA and protein in regeneration bud during the first day after amputation. The data obtained confirm that soon after amputation of the part of the tadpole tail, there occurs the emergence of a structure, to some extend, resembling the early embryonic tail bud.
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| http://dx.doi.org/10.21037/sci-2019-044 | DOI Listing |
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Xvent-2 expression in regenerating tails.
Stem Cell Investig
July 2020
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, Russia.
The tail of tadpole is an excellent model for appendage regeneration studies. We analyzed the distribution pattern of the transcription factor Xvent-2 mRNA and protein in the beginning of the regeneration of tadpole tail stumps after amputation. We revealed the emergence of Xvent-2 mRNA and protein in regeneration bud during the first day after amputation.
View Article and Find Full Text PDFThe proteins of Vent-family and their mRNAs are located in different areas of the tails of Zebrafish and Xenopus embryos.
Int J Biochem Cell Biol
October 2016
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Leninsky Pr. 33, Moscow 119071, Russia. Electronic address:
In molecular embryology, by tacit consent, a presence or absence of the specific mRNA in the cell indicates the presence or absence of the corresponding protein. However, there are lots of evidences that mRNA may be associated with inactive non-translated ribonucleoprotein complexes. Here we for the first time compared the temporal and spatial distribution of the Vent-family transcription factors and their mRNAs in the tails of embryos of two biological species-Xenopus laevis and Zebrafish (Danio reria).
View Article and Find Full Text PDF[Detection of transcription factor Xvent-2 in early development of Xenopus laevis].
Mol Biol (Mosk)
February 2009
The transcription factor Xvent-2 is considered to appear in the embryos of Xenopus after starting of activity of zygote genome, at midblastula stage. But nobody has detected this protein immunologically. All researchers deal with mRNA only.
View Article and Find Full Text PDFPatterning the forebrain: FoxA4a/Pintallavis and Xvent2 determine the posterior limit of Xanf1 expression in the neural plate.
Development
May 2004
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
During early development of the nervous system in vertebrates, expression of the homeobox gene Anf/Hesx1/Rpx is restricted to the anterior neural plate subdomain corresponding to the presumptive forebrain. This expression is essential for normal forebrain development and ectopic expression of Xenopus Anf, Xanf1 (also known as Xanf-1), results in severe forebrain abnormalities. By use of transgenic embryos and a novel bi-colour reporter technique, we have identified a cis-regulatory element responsible for transcriptional repression of Xanf1 that defines its posterior expression limit within the neural plate.
View Article and Find Full Text PDFAnalysis of Spemann organizer formation in Xenopus embryos by cDNA macroarrays.
Dev Biol
May 2004
Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.
The understanding of vertebrate development has greatly benefited from the study of gastrulation in the Xenopus embryo. Over the years, the molecular dissection of the Spemann organizer has proven to be a very fruitful source for gene discovery. Here, we report a comprehensive screen of gene expression in the Xenopus gastrula using cDNA macroarrays.
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