Comprehensive studies of pure colonies of Streptomyces fradiae in the production of xylose isomerase by submerged fermentation at shake flask level revealed poor culture stability with respect to enzyme production, biomass formation, degree of pigmentation, quantity of glucose and xylose utilization, level of enzyme in cell-free culture broth and final pH of the fermentation medium. The results serve to stress obligatory evaluation of culture stability of Streptomyces strains in determining their suitability for use in developing fermentation processes for commercial exploitation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02928009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Improving natural red pigment production by Streptomyces phaeolivaceus strain GH27 for functionalization of textiles with in silico ADME prediction.
BMC Microbiol
January 2025
Microbial Chemistry Department, Biotechnology Research Institute, National Research Center, Dokki, Giza, Egypt.
The red pigment was recovered from the S. phaeolivaceus GH27 isolate, which was molecularly identified using 16S rRNA gene sequencing and submitted to GenBank as OQ145635.1.
View Article and Find Full Text PDFEffects of ORF14 gene on melanin expression, fermentation conditions and properties of melanin production in modified strains.
J Biotechnol
January 2025
Key Laboratory of Green Manufacturing and Biosynthesis of Food Bioactive Substances, China General Chamber of Commerce, School of Food and Health, Beijing Technology and Business University, Beijing 100048 China.
Melanin with antioxidant and antibacterial properties can be used in food, cosmetics, biotechnology, and other fields, but its insolubility become a main challenge hindering for its application. In this study, water-soluble melanin produced by the novel species Streptomyces vilmorinianum YP1 was characterized using scanning electron microscopy (SEM), UVvisible spectroscopy (with an absorption peak at 220 nm), and Fourier transform infrared (FTIR) spectroscopy. The glycosyltransferase gene ORF14 was knocked out, which improved the production of water-soluble melanin by inhibiting competitive pathway.
View Article and Find Full Text PDFDeciphering the driving mechanism of microbial community for rapid stabilization and lignocellulose degradation during waste semi-aerobic bioreactor landfilling with multifunctional microbial inoculum.
Waste Manag
January 2025
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, China. Electronic address:
Owing to the massive refractory lignocellulose and leachate-organic loads, the stabilization of municipal solid waste (MSW) landfill is often prolonged, resulting in environmental burdens. Herein, various assembled multifunctional microbial inoculums (MMIs) were introduced into the semi-aerobic bioreactor landfill (SABL) to investigate the bioaugmentation impacts. Compared to control (CK) and other MMIs treatments (G1-G3), LD + LT + DM inoculation (G4) significantly increased volatile solids degradation (9.
View Article and Find Full Text PDFEnhancement of FK520 production in Streptomyces hygroscopicus var. ascomyceticus ATCC 14891 by overexpressing the regulatory gene fkbR2.
Bioprocess Biosyst Eng
January 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Ascomycin (FK520) is a 23-membered macrolide antibiotic primarily produced by the Streptomyces hygroscopicus var. ascomyceticus. Structurally similar to tacrolimus and rapamycin, it serves as an effective immunosuppressant widely used in the treatment of rejection reactions after organ transplantation and certain autoimmune diseases.
View Article and Find Full Text PDFUnravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity.
Microb Cell Fact
January 2025
Molecular Biology Department, Biotechnology Research Institute, National Research Center, El-Buhouth St. 33, Dokki, P.O.12622, Giza, Egypt.
Background: Actinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial.
Results: In this study, about 34 randomly selected Streptomyces isolates were discovered in soil, sediment, sea water, and other environments.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!