Low-intensity pulsed ultrasound (LIPUS) has been used to accelerate bone fracture healing. However, the issue whether LIPUS is effective in preventing osteoporosis has not been clarified, and if so, what possible mechanisms might be responsible. Myostatin (MSTN) is a negative regulator of muscle growth, and its absence will trigger a positive response to bone. In this study, we examined the effects of LIPUS on bone micro-structure, mechanical properties and damage healing of hindlimb-suspended rats, and investigated whether the inhibition of MSTN plays a role in this process. The rats were randomly divided into four groups: Normal control group (NC), Hind limb suspension group (HLS), Hind limb suspension and 80 mW/cm LIPUS irradiation group (HLS+ 80 mW/cm), Hind limb suspension and 30 mW/cm LIPUS irradiation group (HLS+ 30 mW/cm). The HLS+ 80 mW/cm rats were treated with LIPUS (1 MHz, 80 mW/cm) and the HLS+ 30 mW/cm rats were treated with LIPUS (1 MHz, 30 mW/cm) on the femur for 20 min/day for 28 days. MC3T3-E1 cells were respectively cultured with the serum of wild type mouse and MSTN knockout mouse at 1% concentration for 7 days. After 28 days, LIPUS effectively prevented the destruction of bone microstructure and the decline of mechanical properties, and promoted bone defect healing in the tail-suspended rats. In addition, LIPUS effectively reduced the MSTN content in the quadriceps and serum of the tail-suspended rats, inhibited its receptor and downstream signaling molecules and activated the Wnt signaling pathway in femurs. Growth of MC-3T3-E1 cell cultured with the serum of MSTN knockout mice was superior to that with wild mice serum on day 7. These results indicate that MSTN is a key mediator in LIPUS preventing bone loss caused by hindlimb-suspension.
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http://dx.doi.org/10.1016/j.bone.2020.115610 | DOI Listing |
Chemistry
December 2024
Xinjiang Medical University, State Key Laboratory of Pathogenesis, State Key Laboratory of Pathogenesis, Urumqi, CHINA.
Small molecules with an acceptor-donor-acceptor (A-D-A) structure, featuring a fused-ring core as the donor and two electron-withdrawing end groups as acceptor units, represent a potential option for NIR-II fluorophores, benefiting from their narrow bandgaps, superior light-harvesting capabilities, and exceptional photostabilities. However, their planar conformations predispose them to forming H-aggregates during self-assembly, leading to significantly reduced fluorescence quantum yield (QY) of the resulting nanofluorophores. Herein, we report a small molecule, PF8CN, with a terminal unit-A-D-A-terminal unit structure.
View Article and Find Full Text PDFFASEB J
December 2024
Systems Engineering and Science, Graduate School of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.
Flavan-3-ols (FL) are poorly bioavailable astringent polyphenols that induce hyperactivation of the sympathetic nervous system. The aim of this study was to investigate the effects of repeated oral administration of FL on mice hindlimb skeletal muscle using immunohistochemical techniques. C57BL/6J male mice were orally administered 50 mg/kg of FL for a period of 2 weeks, and bromideoxyuridine (BrdU) was administered intraperitoneally 3 days prior to the dissection.
View Article and Find Full Text PDFJ Clin Lab Anal
December 2024
Department of Laboratory Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Background: Thromboelastography (TEG) and coagulation tests can be used to detect hypercoagulability to assess thrombus formation. This study explored the association between TEG and coagulation in evaluating disease severity in elderly patients with lower extremity arteriosclerotic occlusive disease (LEASO), aimed to provide surgical treatment guideline.
Methods: We retrospectively analyzed the clinical characteristics, laboratory biomarkers, TEG and coagulation parameters of 233 elderly LEASO patients treated between 2020 and 2023.
Muscle Nerve
December 2024
Aix-Marseille Univ, CNRS, CRMBM, Marseille, France.
Introduction And Aims: Mitochondrial myopathies are rare genetic disorders for which no effective treatment exists. We previously showed that the pharmacological cyclophilin inhibitor cyclosporine A (CsA) extends the lifespan of fast-twitch skeletal muscle-specific mitochondrial transcription factor A knockout (Tfam KO) mice, lacking the ability to transcribe mitochondrial DNA and displaying lethal mitochondrial myopathy. Our present aim was to assess whether the positive effect of CsA was associated with improved in vivo mitochondrial energy production.
View Article and Find Full Text PDFJ Orthop
July 2025
Baylor University Medical Center, Department of Orthopaedics, Dallas, TX, USA.
Objective: To perform a systematic review of the utility of exoskeleton robotic therapy on lower extremity recovery in Spinal Cord Injury (SCI) patients.
Methods: We used the Embase, Cochrane, and PubMed databases and searched from to December 2023 for studies on exoskeleton robotic assist devices used in working with SCI patients. Only articles published in English were evaluated, and the retrieved articles were screened via our inclusion/exclusion criteria.
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