Methane is a promising feedstock with high abundance and low cost for the sustainable production of biochemicals and biofuels. Methanotrophic bacteria are particularly interesting platforms for methane bioconversion as they can utilize methane as a carbon substrate. Recently, breakthroughs in the understanding of methane metabolism in methanotrophs as well as critical advances in systems metabolic engineering of methanotrophic bacteria have been reported. Here, we discuss the important gaps in the understanding of methanotrophic metabolism that have been uncovered recently and the current trends in systems metabolic engineering in both methanotrophic bacteria and non-native hosts to advance the potential of methane-based biomanufacturing.
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http://dx.doi.org/10.1016/j.tibtech.2020.07.007 | DOI Listing |
Water Res
January 2025
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, Shandong, China; Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, Shandong, China. Electronic address:
Sulfur-siderite driven autotrophic denitrification (SSAD) has received increasing attention for nutrient removal in constructed wetlands (CWs). Nevertheless, its effectiveness in simultaneous water purification and greenhouse gases (GHGs) reduction remains obscure. In this study, three vertical flow constructed wetlands (VFCWs), filled with quartz sand (CCW), sulfur (S-CW), and sulfur-siderite mixed substrates (SS-CW), were constructed to investigate the underlying mechanisms of SSAD on water purification enhancement and GHGs reduction.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, USA.
Current estimates of wetland contributions to the global methane budget carry high uncertainty, particularly in accurately predicting emissions from high methane-emitting wetlands. Microorganisms drive methane cycling, but little is known about their conservation across wetlands. To address this, we integrate 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux data across 9 wetlands, creating the Multi-Omics for Understanding Climate Change (MUCC) v2.
View Article and Find Full Text PDFBioresour Technol
January 2025
Instituto de Ingeniería, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, C.P. 04510 Ciudad de México, México. Electronic address:
Biological methane oxidation can sustain high temperatures in organic matrices, such as landfill covers and compost biofilters. This study investigates the temperature dynamics, methane removal efficiency, and microbial community responses in a pilot scale compost biofilter under three methane concentrations (2, 4, and 8 % v v in air) with a 23-minute empty bed residence time. Complete methane removal was achieved at 2 %, with compost bed temperatures reaching 51 °C.
View Article and Find Full Text PDFSci Data
January 2025
Division of Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
Permafrost soils store vast amounts of organic carbon, and their thawing due to climate warming accelerates the release of carbon as methane and carbon dioxide, exacerbating global climate change. Understanding the distribution of greenhouse gases trapped in these soils and predicting their behavior upon thawing is essential for accurately modeling climate feedbacks. This study presents an integrated biogeochemical and microbial dataset from ~1.
View Article and Find Full Text PDFCommun Biol
January 2025
University of Chinese Academy of Sciences, 10049, Beijing, China.
Recent studies have unveiled the deep sea as a rich biosphere, populated by species descended from shallow-water ancestors post-mass extinctions. Research on genomic evolution and microbial symbiosis has shed light on how these species thrive in extreme deep-sea conditions. However, early adaptation stages, particularly the roles of conserved genes and symbiotic microbes, remain inadequately understood.
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