While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology. CLIAs and ELISAs can be automated to a high degree but commonly require larger serum or plasma volumes for sample processing. We addressed the suitability of EDTA-anticoagulated whole blood as an alternative sample material for antibody testing against SARS-CoV-2 by electro-CLIA (ECLIA; Roche, Rotkreuz, Switzerland) and ELISA (IgG and IgA; Euroimmun, Germany). Simultaneously drawn venous serum and EDTA-anticoagulated whole blood samples from 223 individuals were included. Correction of the whole blood results for hematocrit led to a good agreement with the serum results for weakly to moderately positive antibody signals. In receiver-operating characteristic curve analysis, all three assays displayed comparable diagnostic accuracy (area under the curve (AUC)) using corrected whole blood and serum (AUCs: 0.97 for ECLIA and IgG ELISA; 0.84 for IgA ELISA). In conclusion, our results suggest that the investigated assays can reliably detect antibodies against SARS-CoV-2 in hemolyzed whole blood anticoagulated with EDTA. Correction of these results for hematocrit is suggested. This study demonstrates that the automated processing of whole blood for identification of SARS-CoV-2 antibodies with common ECLIA and ELISA methods is accurate and feasible.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460437 | PMC |
| http://dx.doi.org/10.3390/diagnostics10080593 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CALR frameshift mutation detection in myeloproliferative neoplasms by microfluidic chip analysis.
Lab Med
December 2024
Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX, United States.
Background: CALR mutation analysis is routinely used to diagnose BCR/ABL1-negative myeloproliferative neoplasms. The 2 most common CALR mutations are a 52-base pair (bp) deletion and a 5-bp insertion, which account for approximately 85% of cases.
Methods: To evaluate our new microfluidic chip assay, we tested CALR mutant and wild-type specimens that were previously analyzed using conventional methods at a reference laboratory.
A rapid and inexpensive 96-well DNA-extraction method from blood using silicon dioxide powder (Glassmilk).
Biol Methods Protoc
October 2024
One Health Research Group, Facultad de Ciencias de la Salud, Universidad de Las Américas, Quito, 170124, Ecuador.
We present a rapid high-throughput DNA extraction method for use with EDTA-anticoagulated blood using silicon dioxide (SiO) powder in a guanidine-HCl solution, hereinafter referred to as "Glassmilk." The method utilizes a 96-well deep-well plate, enabling DNA extraction from 96 samples in under 3 h. The method integrates cell lysis, washing, elution, and DNA storage within the same well, eliminating the need for DNA transfer.
View Article and Find Full Text PDFComparison of basic lymphocyte phenotype results between a diagnostic and a research laboratory.
Lab Med
November 2024
Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles, CA, US.
Objective: Lymphocyte phenotyping is a valuable tool for monitoring the effects of antiretroviral therapy on individuals living with HIV-1. A switch study was conducted to compare T-cell subset quantification performed by a research laboratory and a diagnostic, laboratory to understand the impact on the retrospective and prospective results of a long-term study.
Methods: Using FACSCanto II Flow Cytometers, EDTA anticoagulated peripheral blood from 73 males enrolled in the Multicenter AIDS Cohort Study/Women Interagency HIV Combined Cohort Study was analyzed by both a research (laboratory 1) and a diagnostics laboratory (laboratory 2) for quantification of cluster of differentiation (CD)3, CD4, and CD8 T-cells.
Decreased monocytic HLA-DR in patients with sepsis: Prediction of diagnosis, severity and prognosis.
Clin Biochem
November 2024
Department of Infectious Diseases, The First Affiliated Hospital of Soochow University, Suzhou, China. Electronic address:
Objective: Sepsis is characterized by high incidence and mortality rates, making early recognition and risk stratification critical for preventing delayed treatment and overtreatment. This study investigated the potential of monocytic (m) HLA-DR as a diagnostic and prognostic biomarker of sepsis.
Methods: In this prospective study, we collected blood in EDTA-anticoagulated tubes within 48 h from patients diagnosed with sepsis or infection and analyzed the percentage of mHLA-DR in peripheral blood mononuclear cells, C-reactive protein, and procalcitonin within 2 h of collection.
Insights into Leptospira spp. infection in pet cats in Croatia: Clinical, serological and molecular findings with emphasis on the potential important role of serogroup Pomona.
Acta Trop
December 2024
Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia.
Article Synopsis
- *In a study with pet cats, 18.52% showed antibodies against Leptospira spp., mainly from the Pomona serogroup, while a small percentage had the bacteria DNA present in urine samples.
- *Juxtaposing seropositivity to lifestyle factors revealed outdoor cats were at higher risk, particularly those with other cats in the household and those undergoing immunosuppressive treatment, showcasing a connection to respiratory issues and blood-related conditions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!