AI Article Synopsis
- Researchers developed a method to create stable isotope-labeled internal standards needed for accurate DNA modification analysis using mass spectrometry.
- The synthesis process involves converting [C,N]-2'-deoxythymidine into [C,N]-5-methyl-2'-deoxycytidine and further oxidizing it to form various derivatives.
- Optimized protocols were also established for oxidizing [C,N]-thymine into several compounds, enhancing the potential for precise quantitative analysis in DNA studies.
Article Abstract
Reliable quantitative analysis of DNA modification using liquid chromatography coupled with tandem mass spectrometry requires stable isotope-labeled internal standards. Only some of them are commercially available. Here we present a method allowing for the synthesis of [C,N]-5-methyl-2'-deoxycytidine from [C,N]-2'-deoxythymidine. We also describe an approach for the oxidation of [C,N]-5-methyl-2'-deoxycytidine and [C,N]-2'-deoxythymidine with NaSO, leading to the generation of [C,N]-5-formyl-2'-deoxycytidine, [C,N]-5-carboxy-2'-deoxycytidine or [C,N]-5-(hydroxymethyl)-2'-deoxyuridine, correspondingly. Moreover, we provide optimized protocols for the oxidation of [C,N]-thymine to [C,N]-5-hydroxymethyluracil, [C,N]-5-formyluracil, and [C,N]-5-carboxyuracil using NaSO.
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| http://dx.doi.org/10.1007/978-1-0716-0876-0_10 | DOI Listing |
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