Aim: To investigate the effects of hydrogen (H) on Cu, Zn superoxide dismutase (SOD1) activation in a rat model of corneal alkali burn.
Methods: In each rat, one cornea was subjected to alkali exposure. Physiological saline (saline group) or H-dissolved saline (H group) was instilled continuously on the cornea for 5min before and after alkali exposure. Inflammatory cells, neovascularization, and cytoplasmic SOD1 levels were evaluated immunohistochemically in enucleated eyes from both groups. Three-dimensional ultrastructural tissue changes in the eyes were analyzed using low-vacuum scanning electron microscopy.
Results: The numbers of both inflammatory and vascular endothelial cells were significantly reduced in the corneas of the H group (<0.01). Furthermore, H treatment increased both cytoplasmic SOD1 levels (<0.01) and activity in corneal epithelial cells (<0.01). Notably, the SOD1 activity level in the H group was approximately 2.5-fold greater than that in the saline group.
Conclusion: H treatment suppresses inflammation and neovascularization in the injured cornea and indirectly suppresses oxidative insult to the cornea by upregulating the SOD1 enzyme protein level and activity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387904 | PMC |
| http://dx.doi.org/10.18240/ijo.2020.08.01 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of the anti-inflammatory, antioxidant and wound healing effects of pterostilbene in human gingival fibroblasts in vitro.
Odontology
January 2025
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
We aimed to investigate the wound-healing, antioxidant, and anti-inflammatory effects of pterostilbene (PTS) on human gingival fibroblasts (GF). Different concentrations of PTS were applied to GFs and cell viability was evaluated by MTT assay. GFs were stimulated by lipopolysaccharide (LPS) and the study groups were determined as LPS, LPS + 1 μM PTS, LPS + 10 μM PTS, and control.
View Article and Find Full Text PDFMitigation of Neuroinflammation and Oxidative Stress in Rotenone-Induced Parkinson Mouse Model through Liposomal Coenzyme-Q10 Intervention: A Comprehensive In-vivo Study.
Inflammation
January 2025
Department of Chemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Parkinson's disease (PD) stands as the sec most prevalent incapacitating neurodegenerative disorder characterized by deterioration of dopamine-producing neurons in the substantia nigra. Coenzyme Q10 (CoQ10) has garnered attention as a potential antioxidant, anti-inflammatory agent and enhancer of mitochondrial complex-I activity. This study aimed to examine and compare the effectiveness of liposomal and non-encapsulated CoQ10 in rotenone induced-PD mouse model over a 21-day treatment duration.
View Article and Find Full Text PDFEffect of hydro-ethanol extract of Caesalpinia pulcherrima (L.) Sw. leaves in human and rat: In vitro approach of male contraceptive development.
JBRA Assist Reprod
January 2025
Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Objective: The study focused the contraceptive efficacy of hydro-ethanolic (60:40) extract (HEE) of Caesalpinia pulcherrima leaves in human and rat sperm samples by in vitro study.
Methods: Six young fertile adult males were selected for semen collection. Sperm samples were collected from six adult rat also by chopping the epididymis along with the collection of testicles, epididymis, and liver.
Prussian Blue Nanozyme Featuring Enhanced Superoxide Dismutase-like Activity for Myocardial Ischemia Reperfusion Injury Treatment.
ACS Nano
January 2025
Jiangsu Key Laboratory for Biomaterials and Devices, School of Biomedical Sciences and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
The blood flow, when restored clinically following a myocardial infarction (MI), disrupts the physiological and metabolic equilibrium of the ischemic myocardial area, resulting in secondary damage termed myocardial ischemia-reperfusion injury (MIRI). Reactive oxygen species (ROS) generation and inflammatory reactions stand as primary culprits behind MIRI. Current strategies focusing on ROS-scavenging and anti-inflammatory actions have limited remission of MIRI.
View Article and Find Full Text PDFOptimizing soil health through activated acacia biochar under varying irrigation regimes and cultivars for sustainable wheat cultivation.
PeerJ
January 2025
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.
Wheat, a staple food crop globally, faces the challenges of limited water resources and sustainable soil management practices. The pivotal elements of the current study include the integration of activated acacia biochar (AAB) in wheat cultivation under varying irrigation regimes (IR). A field trial was conducted in the Botanical Garden, University of the Punjab, Lahore during 2023-2024, designed as a split-split-plot arrangement with RCBD comprising three AAB levels (0T, 5T, and 10T, T = tons per hectare) three wheat cultivars (Dilkash-2020, Akbar-2019, and FSD-08) receiving five IR levels (100%, 80%, 70%, 60%, and 50% field capacity).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!