sp. nov., an endogenous actinomycete isolated from .

Int J Syst Evol Microbiol

Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.

Published: October 2020

AI Article Synopsis

  • A new strain of actinobacteria, named TRM 66187, was isolated from Alar, Xinjiang, and studied using a detailed taxonomic method.
  • The strain shows high genetic similarity (98.48%) to a known species, DSM 42035, and has distinct biochemical properties, such as specific sugar patterns and fatty acids.
  • Based on the research findings, TRM 66187 is proposed as a new species in its genus, with the type strain designated for future reference.

Article Abstract

A novel endogenous actinobacteria strain, designated TRM 66187, was isolated from sampled at Alar, Xinjiang, Northwest PR China, and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66187 with the genus . The whole-cell sugar pattern of TRM 66187 consisted of galactose, glucose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H). Major cellular fatty acids were iso-C, iso-C, anteiso-C and anteiso-C. The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified polar lipids. The G+C content of strain TRM 66187 was 71.8 mol%. Results of phylogenetic analysis showed that strain TRM 66187 had 98.48% 16S rRNA gene sequence similarity to the closest described species DSM 42035. The average nucleotide identity value between strain TRM 66187 and the closest related strain DSM 42035 was calculated to be 77.2%. The digital DNA-DNA hybridization value between them was 22.4%. Multilocus sequence analyses based on five housekeeping genes (, , , and ) also indicated that strain TRM 66187 should be assigned to the genus . On the basis of evidence from this polyphasic study, strain TRM 66187 should be designated as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 66187 (=LMG 31493=CCTCC AA 2018094).

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http://dx.doi.org/10.1099/ijsem.0.004372DOI Listing

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Article Synopsis
  • A new strain of actinobacteria, named TRM 66187, was isolated from Alar, Xinjiang, and studied using a detailed taxonomic method.
  • The strain shows high genetic similarity (98.48%) to a known species, DSM 42035, and has distinct biochemical properties, such as specific sugar patterns and fatty acids.
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