A novel endogenous actinobacteria strain, designated TRM 66187, was isolated from sampled at Alar, Xinjiang, Northwest PR China, and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66187 with the genus . The whole-cell sugar pattern of TRM 66187 consisted of galactose, glucose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H). Major cellular fatty acids were iso-C, iso-C, anteiso-C and anteiso-C. The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified polar lipids. The G+C content of strain TRM 66187 was 71.8 mol%. Results of phylogenetic analysis showed that strain TRM 66187 had 98.48% 16S rRNA gene sequence similarity to the closest described species DSM 42035. The average nucleotide identity value between strain TRM 66187 and the closest related strain DSM 42035 was calculated to be 77.2%. The digital DNA-DNA hybridization value between them was 22.4%. Multilocus sequence analyses based on five housekeeping genes (, , , and ) also indicated that strain TRM 66187 should be assigned to the genus . On the basis of evidence from this polyphasic study, strain TRM 66187 should be designated as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 66187 (=LMG 31493=CCTCC AA 2018094).
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http://dx.doi.org/10.1099/ijsem.0.004372 | DOI Listing |
Int J Syst Evol Microbiol
March 2022
Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, PR China.
A novel actinomycete, strain 1_25, was isolated from soil under a black Gobi rock sample from Shuangta, PR China, and characterized using a polyphasic taxonomic approach. The results of comparative analysis of the 16S rRNA gene sequences indicated the 1_25 represented a member of the genus . Chemotaxonomic data revealed that 1_25 possessed MK-9(H) as the major menaquinone.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
October 2020
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
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