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Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation. | LitMetric

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation.

J Biol Chem

Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

Published: October 2020

AI Article Synopsis

Article Abstract

Among the multiple antiviral defense mechanisms found in prokaryotes, CRISPR-Cas systems stand out as the only known RNA-programmed pathways for detecting and destroying bacteriophages and plasmids. Class 1 CRISPR-Cas systems, the most widespread and diverse of these adaptive immune systems, use an RNA-guided multiprotein complex to find foreign nucleic acids and trigger their destruction. In this review, we describe how these multisubunit complexes target and cleave DNA and RNA and how regulatory molecules control their activities. We also highlight similarities to and differences from Class 2 CRISPR-Cas systems, which use a single-protein effector, as well as other types of bacterial and eukaryotic immune systems. We summarize current applications of the Class 1 CRISPR-Cas systems for DNA/RNA modification, control of gene expression, and nucleic acid detection.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573268PMC
http://dx.doi.org/10.1074/jbc.REV120.007034DOI Listing

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