The bioaccumulation of cadmium (Cd) in crop and the subsequent food chain has aroused extensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance remain to be clarified from the viewpoint of novel candidate genes. Here we described a highly efficient approach for preliminary identifying rice Cd-tolerant genes through the yeast-based cDNA library survival screening combined with high-throughput sequencing strategy. About 690 gene isoforms were identified as being Cd-tolerant candidates using this shotgun approach. Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves. Regarding the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated domain (HMAD) isoprenylated protein HIPP42 was particularly noteworthy. Further elucidation of these genes associated with Cd tolerance in rice will benefit agricultural activities.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.plaphy.2020.07.046 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Overexpression Enhances Cadmium Tolerance in .
Environ Sci Technol
December 2024
Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, Guizhou, China.
Glutathione S-transferase (GST) has been established to play an important role in regulating the responses of plants to stress, although its function and mechanisms of action in the cadmium (Cd)-tolerant remain unclear. In this study, we sought to identify a Cd-responsive gene from for functional analysis and mechanistic characterization. We accordingly identified a member of the gene family, , which plays a positive role in adaptation of to Cd.
View Article and Find Full Text PDFArbuscular Mycorrhizal Fungi-Assisted Phytoremediation: A Promising Strategy for Cadmium-Contaminated Soils.
Plants (Basel)
November 2024
Guangdong Engineering and Technology Center for Environmental Pollution Prevention and Control in Agricultural Producing Areas, College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Arbuscular mycorrhizal fungi (AMF) have been shown to play a major role in regulating the accumulation, transport, and toxicity of cadmium (Cd) in plant tissues. This review aims to highlight the current understanding of the mechanisms by which AMF alleviate Cd toxicity in plants. Cd accumulation in agricultural soils has become an increasing global concern due to industrial activities and the use of phosphatic fertilizers.
View Article and Find Full Text PDFExploring genotypic variation and gene expression associated to cadmium accumulation in bread wheat.
Sci Rep
November 2024
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Cadmium (Cd) contamination poses significant risks to agricultural productivity and human health, particularly through its accumulation in staple crops such as bread wheat (Triticum aestivum L.). This study evaluated Cd accumulation and tolerance among six bread wheat cultivars exposed to six Cd concentrations (0, 2.
View Article and Find Full Text PDFDNA-stable isotope probing and metagenomics reveal Fe(II) oxidation by core microflora in microoxic rhizospheric habitats to mitigate the accumulation of cadmium and phenanthrene in rice.
Environ Pollut
December 2024
College of New Energy and Environment, Jilin University, Changchun, 130021, China.
Rice rhizosphere soil-porewater microdomains exist within an iron (Fe)-rich microoxic habitat during paddy soil flooding. However, the response mechanisms of core microflora in this habitat to Fe(II)-oxidation-mediated cadmium (Cd) and phenanthrene (Phen) remain unclear. Using gel-stabilized gradient systems to replicate the microoxic conditions in the rice rhizosphere porewater, we found that microaerophilic rhizobacteria drove Fe(II) oxidation to yield iron oxides, thereby reducing the Cd and Phen contents in the rhizosphere porewater and rice (Cd and Phen decreased by 15.
View Article and Find Full Text PDFResponses of Brassica napus to soil cadmium under elevated CO concentration based on rhizosphere microbiome, root transcriptome and metabolome.
Plant Physiol Biochem
November 2024
Center of Molecular Ecophysiology (CMEP), College of Resources and Environment, Southwest University, Chongqing, 400715, PR China. Electronic address:
Article Synopsis
- * Exposure to ECO increased Cd uptake in B. napus by nearly 39%, improving plant biomass and leaf chlorophyll content significantly compared to Cd exposure alone.
- * The study found that ECO led to enhanced antioxidant activity and changes in rhizosphere microbes, indicating potential regulatory mechanisms for stress responses, which could aid in developing Cd-tolerant rapeseed varieties for future climates.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!