Investigating the multimolecule patterns in living cells is of vital importance for clinical and biomedical studies. Herein, we reported for the first time the engineering of gold nanoflares as smart automata to implement computing-based diagnosis in living mammalian cells. Defining the logic combinations of miR122 and miR21 as the detection patterns, the corresponding OR and AND diagnostic automata were designed. The results showed that they could recognize the correct patterns rapidly and sensitively. The automata could enter cells via self-delivery and have good biocompatibility. They enabled accurate diagnosis on miRNA signatures in different cell lines and differentiation of fluctuations in the same cell line at single cell resolution. Moreover, the automata afforded an innovative diagnostic mode. It simplified the complicated process of detecting, data-collecting, computing, and evaluating. The direct diagnosing result ("1" or "0") was exported according to the embedded computation code. It highlighted the new possibility of using smart automata for intelligent diagnostics and cancer therapy at single cell resolution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.0c02325 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Towards the Development of an Optical Biosensor for the Detection of Human Blood for Forensic Analysis.
Sensors (Basel)
November 2024
Department of Analytical, Environmental & Forensic Sciences, King's College London, London SE1 9NH, UK.
Blood is a common biological fluid in forensic investigations, offering significant evidential value. Currently employed presumptive blood tests often lack specificity and are sample destructive, which can compromise downstream analysis. Within this study, the development of an optical biosensor for detecting human red blood cells (RBCs) has been explored to address such limitations.
View Article and Find Full Text PDFPt-Decorated Gold Nanoflares for High-Fidelity Phototheranostics: Reducing Side-Effects and Enhancing Cytotoxicity toward Target Cells.
Angew Chem Int Ed Engl
May 2024
Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, and School of Food and Bioengineering, Changsha University of Science and Technology, Changsha, 410114, China.
Functionalized with the Au-S bond, gold nanoflares have emerged as promising candidates for theranostics. However, the presence of intracellular abundantly biothiols compromises the conventional Au-S bond, leading to the unintended release of cargoes and associated side-effects on non-target cells. Additionally, the hypoxic microenvironment in diseased regions limits treatment efficacy, especially in photodynamic therapy.
View Article and Find Full Text PDFMonitoring and Regulating Intracellular GPX4 mRNA Using Gold Nanoflare Probes and Enhancing Erastin-Induced Ferroptosis.
Biosensors (Basel)
December 2022
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
Glutathione peroxidase 4 (GPX4) plays an important effect on ferroptosis. Down-regulating the expression of GPX4 mRNA can decrease the content of GPX4. In this work, a gold nanoflare (AuNF) probe loaded with anti-sense sequences targeting for GPX4 mRNA was designed to monitor and down-regulate intracellular GPX4 mRNA using fluorescence imaging in situ and using anti-sense technology.
View Article and Find Full Text PDFCirculating CD133+/–CD34– Have Increased c- Expression in Myeloproliferative Neoplasms.
Turk J Haematol
February 2023
İstanbul University Aziz Sancar Institute of Experimental Medicine, Department of Genetics, İstanbul, Türkiye
Objective: Myeloproliferative neoplasms (MPNs) are hematopoietic stem cell (HSC)-originated diseases with clonal myeloproliferation. The constitutive activation of the JAK/STAT pathway is frequently detected in patients with Philadelphia chromosome-negative (Ph–) MPNs with an acquired V617F mutation. The c- proto-oncogene is associated with malignant growth and cellular transformation, and V617F was previously shown to induce constitutive expression of c-.
View Article and Find Full Text PDFUltra-thin silica shell-guarded nanoflares for high-fidelity live cell miRNA-21 imaging by fully avoiding the interference of biothiols.
Analyst
November 2022
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, P.R. China.
We successfully constructed a new class of nanoflares based on ultra-thin silica-coated gold nanoparticles (Au@SiO) with the covalent binding of nucleic acids, which demonstrated more resistance to biothiols than that exhibited in the traditional Au-S binding strategy, for imaging the target miRNA-21 with high fidelity in living cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!