Metabolic and Process Engineering of for Butyl Acetate Production in One Step.

-Butyl acetate is an important food additive commonly produced concentrated sulfuric acid catalysis or immobilized lipase catalysis of butanol and acetic acid. Compared with chemical methods, an enzymatic approach is more environmentally friendly; however, it incurs a higher cost due to lipase production. biosynthesis metabolic engineering offers an alternative to produce -butyl acetate. This alternative combines substrate production (butanol and acetyl-coenzyme A (acetyl-CoA)), alcohol acyltransferase expression, and esterification reaction in one reactor. The alcohol acyltransferase gene ATF1 from was introduced into NCIMB 8052, enabling it to directly produce -butyl acetate from glucose without lipase addition. Extractants were compared and adapted to realize glucose fermentation with -butyl acetate extraction. Finally, 5.57 g/L of butyl acetate was produced from 38.2 g/L of glucose within 48 h, which is 665-fold higher than that reported previously. This demonstrated the potential of such a metabolic approach to produce -butyl acetate from biomass.