Tryptophol Coating Reduces Catheter-Related Cerebral and Pulmonary Infections by .

Infect Drug Resist

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Published: July 2020

AI Article Synopsis

  • Central venous catheters (CVCs) are prone to severe complications from fungal colonization, specifically by an opportunistic pathogen, but a quorum-sensing molecule called tryptophol (TOH) has shown potential in controlling this issue.
  • In experiments, TOH demonstrated a dose-dependent ability to inhibit pathogen growth and significantly reduced biofilm formation and fungal cell viability on catheter surfaces.
  • Coating catheters with TOH not only mitigated cerebral and lung damage associated with infections but also enhanced survival rates in experimental models, suggesting it as a viable strategy for preventing catheter-related infections.

Article Abstract

Introduction: Central venous catheter (CVC) is a medical device that is used to administer medication for a long duration. Colonization by an emerging opportunistic pathogen in the CVC lumen is frequently reported to cause severe complications in patients. Here, we describe the effect of fungal quorum-sensing molecule (QSM) known as tryptophol (TOH) to control colonization in catheter tube lumens in both in vitro and in vivo models.

Methods: Antifungal susceptibility of TOH against was compared with voriconazole, and the colony diameter was determined on days 2, 4, and 6. Experimental catheterization rat model was conducted with pre-coating of TOH and voriconazole or an uncoated control and an infection with . Biofilm formation on the catheter luminal surface was assessed using the scanning electron microscopy, crystal violet, and 2,3-bis(2-methoxy-4-ni-tro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetra-zolium hydroxide (XTT) reduction assays. Brain and lung samples of catheterized rats were histopathologically assessed. Serum samples from catheterized rats were injected into larvae. Survival of catheterized rats and was determined.

Results: TOH impeded the growth of by reducing the colony diameter in a dose-dependent manner. TOH coating remarkably lessened biofilm formation and fungal cell viability on the catheter luminal surface. Additionally, TOH coating lessens cerebral edema that is associated with abscess and invasive pulmonary damages due to catheter-related infection. Furthermore, TOH coating also lessened the virulence of in sera of experimental catheterized rats and extended the survival rate of larvae infection model.

Conclusion: An alternative modification of catheter by coating with TOH is effective in preventing colonization in vivo. Our study gives a new strategy to control catheter contamination and prevents nosocomial diseases due to infection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383110PMC
http://dx.doi.org/10.2147/IDR.S255489DOI Listing

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