An aptasensor is reported for the detection of three different kinds of mycotoxins, i.e., zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (FB1). Based on fluorescence resonance energy transfer effect (FRET) and surface-enhanced Raman scattering (SERS), the levels of ZEN, FB1, and OTA can be simultaneously determined. Under 980-nm and 650-nm laser excitation, the logarithmic values of fluorescence signal intensities at 543 nm and 670 nm are slowly increased as the concentrations of ZEN and OTA vary from 0.1 ng mL and 0.05 ng mL to 100 ng mL and 25 ng mL, respectively. For FB1, under 980-nm laser excitation, the logarithmic value of SERS signal intensity at 1567 cm gradually increases with the concentration of FB1 in the range 0.05-200 pg mL (R = 0.996). The detection limits of the proposed assay for ZEN, OTA, and FB1 are 0.03 ng mL, 0.01 ng mL, and 0.02 pg mL, respectively. The selectivity experiment results indicate this assay possesses a high selectivity over other commonly encountered mycotoxins. The average recoveries range from 90 to 107%, revealing satisfactory application potential of the proposed assay. The developed aptasensor will bring bright prospects for research in the field of multiplexed mycotoxine detection. Graphical Abstract Schematic representation of an aptamer-based assay for multiple mycotoxins determination.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s00604-020-04487-1 | DOI Listing |
Methods
January 2025
CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal; LABBELS - Associate Laboratory, University of Minho, Braga/Guimarães, Portugal. Electronic address:
Measurements of changes in fluorescence signal is one of the most commonly applied methods for studying protein-ligand affinities. These measurements are generally carried out using cuvettes in spectrofluorometers, which can only measure one sample at a time. This makes screening procedures for multiple ligands and proteins extremely laborious, as each protein must be measured with multiple ligand concentrations, and usually in triplicate.
View Article and Find Full Text PDFAntioxidants (Basel)
December 2024
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Aflatoxins (AFTs) are a form of mycotoxins mainly produced by and , which are common contaminants in various agricultural sources such as feed, milk, food, and grain crops. Aflatoxin B1 (AFB1) is the most toxic one among all AFTs. AFB1 undergoes bioactivation into AFB1-8,9-epoxide, then leads to diverse harmful effects such as neurotoxicity, carcinogenicity, hepatotoxicity, reproductive toxicity, nephrotoxicity, and immunotoxicity, with specific molecular mechanisms varying in different pathologies.
View Article and Find Full Text PDFNPJ Sci Food
December 2024
International Joint Research Center on Food Security (IJC-FOODSEC), Khlong Luang, Pathum Thani, 12120, Thailand.
Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent.
View Article and Find Full Text PDFToxins (Basel)
December 2024
Division of Toxicology, Institute for Medical Research and Occupational Health, HR-10 000 Zagreb, Croatia.
The increasing use of products for medicinal, dietary, and recreational purposes has raised concerns about mycotoxin contamination in cannabis and hemp. Mycotoxins persist in these products' post-processing, posing health risks via multiple exposure routes. This study investigated cytotoxic and genotoxic interactions between cannabidiol (CBD) and the mycotoxin citrinin (CIT) using human cell models: SH-SY5Y, HepG2, HEK293, and peripheral blood lymphocytes.
View Article and Find Full Text PDFToxins (Basel)
November 2024
Department of Obstetrics and Gynecology, Semmelweis University, 1088 Budapest, Hungary.
The effect of mycotoxin exposure on follicular fluid composition and reproductive outcomes in women undergoing in vitro fertilisation (IVF) was investigated in this study. Twenty-five patients were included, and follicular fluid and serum samples were analysed for various mycotoxins. Principal observations:1.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!