AI Article Synopsis
- The crude polysaccharides (GFP) were extracted from the Maitake mushroom and purified using two chromatography methods, resulting in five main fractions labeled GFP-1 to GFP-5.
- Free sugars, identified as glucose and trehalose, were isolated and analyzed through NMR, revealing predominant β-linkages in the polysaccharides.
- The study indicated structural similarities between water soluble and water insoluble polysaccharides, suggesting that the soluble forms may originate from the breakdown of the cell wall.
Article Abstract
The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). GC-MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum. Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown.
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| http://dx.doi.org/10.1016/j.ijbiomac.2020.08.037 | DOI Listing |
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