Control of Antimicrobials in Feed Using Liquid Chromatography-Tandem Mass Spectrometry: Assessment of Cross-Contamination Rates at the Farm Level.

Antimicrobial cross-contamination of animal feed may occur during feed manufacturing, because shared production lines can be used for the production of medicated and nonmedicated feeds, and also during feed transport, storage at the farm level, and usage. This is a major issue in the current context in which antimicrobial usage must be controlled to maintain their effectiveness. The purpose of this study was to assess the antimicrobial cross-contamination rate of feed at the farm level. Here, we optimized a liquid chromatography-tandem mass spectrometry method for the determination of 11 antimicrobials in feed for pigs, poultry, and rabbits, which were strategically chosen. The method was validated according to European regulations in terms of mass spectrometry identification criteria and quantification criteria (linearity, trueness, precision, limit of quantification, and limit of decision). The results were in compliance with these regulations except for doxycycline, which may be quantified with higher uncertainty. This method was applied to the analysis of 192 nonmedicated pig, poultry, and rabbit feed samples that were collected directly from farms to assess antimicrobials animal exposure. Cross-contamination rates were relatively high with 44% of the samples being contaminated at a concentration above the quantification limit of 0.125 mg/kg and 15% of the samples being contaminated above 1 mg/kg. This result suggests that the current regulations and feed processing recommendations need to be improved, taking into account the risks arising from these contaminations.