The study was aimed to investigate the effect of alpha-lipoic acid (ALA) on human umbilical vein endothelial cells (HUVECs) injury induced by hydrogen peroxide (HO) and to explore its possible mechanisms. We established the HO-induced HUVECs injury model and the ALA treatment groups in which HUVECs were co-incubated with HO (250 μmol/L) and different final concentrations of ALA (100,200,400 μmol/L) for 48 h. Cell survival rate assay and LDH activity assay were carried out. The levels of related proteins were performed by Western Blot. We observed that HO administration resulted in an increase in the LDH activity and a decrease in cell survival rate. The expression levels of Nox4, Bax, NF-κB p65, Caspase-9, Caspase-3, iNOS, VCAM-1 and ICAM-1 were up-regulated, while the expression level of Bcl-2 was down-regulated. All these factors were significantly improved by ALA treatment. In brief, ALA treatment ameliorates HO-induced HUVECs damage by inhibiting inflammation and oxidative stress.
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| http://dx.doi.org/10.1080/09168451.2020.1802221 | DOI Listing |
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