Suspended specimens of 2D crystals and their heterostructures are required for a range of studies including transmission electron microscopy (TEM), optical transmission experiments, and nanomechanical testing. However, investigating the properties of laterally small 2D crystal specimens, including twisted bilayers and air-sensitive materials, has been held back by the difficulty of fabricating the necessary clean suspended samples. Here we present a scalable solution that allows clean free-standing specimens to be realized with 100% yield by dry-stamping atomically thin 2D stacks onto a specially developed adhesion-enhanced support grid. Using this new capability, we demonstrate atomic resolution imaging of defect structures in atomically thin CrBr, a novel magnetic material that degrades in ambient conditions.
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http://dx.doi.org/10.1021/acs.nanolett.0c02346 | DOI Listing |
Rev Sci Instrum
December 2024
Atomic and Molecular Physics Division, Bhabha Atomic Research Centre, Mumbai 400094, India.
We present a comprehensive overview of the commissioning process and initial results of a synchrotron beamline dedicated to atomic, molecular, and optical sciences at the BL-5 undulator port of the Indus-2 synchrotron facility, Raja Ramanna Center for Advanced Technology, Indore, India. The beamline delivers a photon flux of ∼1012 photons/s with high resolving power (∼10 000) over an energy range of 6-800 eV, making it suitable for high-resolution spectroscopy in atomic, molecular, and optical science. The energy tunability from vacuum ultraviolet to soft x-ray (6-800 eV) is achieved through a varied line spacing plane grating monochromator with four gratings: very low energy (VLEG), low energy (LEG), medium energy (MEG), and high energy (HEG).
View Article and Find Full Text PDFNucleic Acids Res
December 2024
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
X-ray crystallography is a fundamental technique that provides atomic-level insights into RNA structures. However, obtaining crystals of RNA structures diffracting to high resolution is challenging. We introduce a simple strategy to enhance the resolution limit of RNA crystals by the selective substitution of Watson-Crick pairs by GU pairs within RNA sequences.
View Article and Find Full Text PDFAdv Mater
December 2024
Beijing Key Laboratory of Theory and Technology for Advanced Batteries Materials, School of Materials Science and Engineering, Peking University, Beijing, 100871, China.
Developing sufficiently effective non-precious metal catalysts for large-current-density hydrogen production is highly significant but challenging, especially in low-voltage hydrogen production systems. Here, we innovatively report high-entropy alloy nanoflower array (HEANFA) electrodes with optimizable reaction pathways for hydrazine oxidation-assisted hydrogen production at industrial-grade current densities. Atomic-resolution structural analyses confirm the single-phase solid-solution structure of HEANFA.
View Article and Find Full Text PDFbioRxiv
December 2024
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.
Human RAD52 is a prime target for synthetical lethality approaches to treat cancers with deficiency in homologous recombination. Among multiple cellular roles of RAD52, its functions in homologous recombination repair and protection of stalled replication forks appear to substitute those of the tumor suppressor protein BRCA2. However, the mechanistic details of how RAD52 can substitute BRCA2 functions are only beginning to emerge.
View Article and Find Full Text PDFIn cardiac muscle, many myosin molecules are in a resting or "OFF" state with their catalytic heads in a folded structure known as the interacting heads motif (IHM). Many mutations in the human β-cardiac myosin gene that cause hypertrophic cardiomyopathy (HCM) are thought to destabilize (decrease the population of) the IHM state. The effects of pathogenic mutations on the IHM structural state are often studied using indirect assays, including a single-ATP turnover assay that detects the super-relaxed (SRX) biochemical state of myosin functionally.
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