AI Article Synopsis
- A new amperometric biosensor was created using electropolymerization of L-aspartic acid on a carbon-paste electrode for measuring glucose levels, employing cyclic voltammetry.
- The modified electrode enhanced glucose oxidase immobilization and improved the biosensor's electrocatalytic performance with a sensitivity of 5.3 µA cmmM and a linear detection range from 0.05 mM to 1.0 mM.
- Additionally, the biosensor exhibited a low limit of detection at 69.2 µM, a Michaelis-Menten constant of 1.17 mM, and maintained good stability and anti-interference capability against substances like dopamine and uric acid.
Article Abstract
A new amperometric biosensor was fabricated by means of electropolymerization of L-aspartic acid on a carbon-paste electrode (CPE) for the bioelectrochemical determination of glucose. The electropolymerization process was conducted via cyclic voltammetry (CV). The modified CPE with poly (L-aspartic acid) (PAA) provided free carboxyl groups so as to immobilize the glucose oxidase (GOx), and further, enhanced the electrocatalytic activity of the hydrogen peroxide (HO). The biosensor displayed both good stability and good bioactivity. The sensitivity of the prepared biosensor was 5.3 µA cmmM. Its linear range extended from 0.05 mM to 1.0 mM, with the low limit of detection (LOD) being 69.2 µM. The Michaelis-Menten constant was found to be 1.17 mM. Furthermore, the biosensor showed good anti-interference ability in relation to dopamine, uric acid, and ascorbic acid. Taken together, these results demonstrate that PAA/CPE is a promising material for the fabrication of glucose biosensor.
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| http://dx.doi.org/10.1080/10826068.2020.1805758 | DOI Listing |
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