Integrative Analysis of lncRNAs in Kidney Cancer to Discover A New lncRNA () as A Therapeutic Target for Staphylococcal Enterotoxin Gene.

Objective: Bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding RNAs (lncRNAs) and genes. In the present study, the effect of gene on ACHN cell lines was reported along with proposing a novel pathway of apoptosis in kidney cancer.

Materials And Methods: In this experimental study, effective lncRNAs and genes were predicted from different criteria for renal cell carcinoma (RCC) by bioinformatics methods and lncRNA-miRNA-mRNA interaction was constructed; then the effect of Staphylococcus aureus tst gene on induction of apoptosis pathways on ACHN and HDF cell lines was investigated.

Results: After creation of lncRNA-miRNA-mRNA interaction, changes in expression levels of lncRNA (P=0.0024) and gene (P=0.0027) were identified, as potential apoptosis biomarkers for kidney cancer, after treating ACHN cell line by pCDNA3.1 (+) compared to the empty vector. In contrast, there was no statistically significant difference in expression levels in ACHN cell (P≥0.05). In addition, transfection by pcDNA3.1 (+) could increase ACHN cell apoptosis level (P<0.0001) compared to the pcDNA3.1 (+) group; but no significant effect was observed on normal cells.

Conclusion: It is suggested that lncRNA LINC00847, discovered in this study, could provide a new landscape for researches aimed to determine relationship between functional lncRNA and RCC pathways. pcDNA3.1 (+) was found to increase apoptosis in the transfected cells.