Effects of different culture media on physiological features and laboratory scale production cost of .

Biotechnol Rep (Amst)

Biochemistry and Molecular Biology Department, Federal University of Paraná, Av. Cel. Francisco H. dos Santos, 100, CEP 81531-980, PO BOX 19046, Curitiba, Brazil.

Published: September 2020

Microalgal cultivation system depends on optimal parameters obtained from laboratory conditions to overcome high-value compounds production and decrease cost. In this study, the laboratory-scale cultivation of was performed to explore the cost and biochemical effects on biomass cultivated using F/2, Conway, and Johnson media. cultivation was monitored by cell counting, dry biomass measurements and biochemical analyses. Photosynthetic pigment profiles were identified and quantified through high-performance liquid chromatography (HPLC) whereas economic evaluation was based on media cost. Cellular growth parameters were significantly improved by Conway medium, whereas maximum accumulation of pigments, especially violaxanthin (0.855 mg g) and chlorophyll (14.255 mg g) were observed when Johnson medium was used. Conway-biomass showed 43 % of total lipid content. This value represents 1.5 and 1.4 times higher than lipid content found in Johnson- and F/2-biomass, respectively. Furthermore, media cost presented a variation of US$1.17-49.62 for each 10 L of media mainly due to NaNO, KNO and cyanocobalamin supplementation. Thus, biomass production showed a cost variation of US$4.64-301.61 per kg on a dry weight basis. These outcomes emphasize the suitability of laboratory parameters and cost comparison among biomass produced by different media.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399117PMC
http://dx.doi.org/10.1016/j.btre.2020.e00508DOI Listing

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