Tailored-design of electrospun nanofiber cellulose acetate/poly(lactic acid) dressing mats loaded with a newly synthesized sulfonamide analog exhibiting superior wound healing.

To effectively allow for controlled release of a newly synthesized sulfonamide analog, biodegradable poly(lactic acid) nanofibrous dressing mats tailored-designed for maximum wound healing efficacy were developed. The heterocyclic analog, N-(3,4-diamino-7-(benzo [d]thiazol-2-yl)-6-oxo-1H-pyrazolo[4,3-c]pyridin-5(6H)-yl)benzenesulfonamide, has been specifically synthesized to possess superior antibacterial and anti-inflammatory characteristics. Hydrophilic cellulose acetate and/or poly(ethylene oxide) were blended with the hydrophobic PLA to control the hydrophilicity/hydrophobicity ratio for the sustained release of the drug. SEM detected no drug crystals on the surface of the nanofibers confirming the homogeneous dispersion and compatibility of the drug with the nanofibers. BET indicated almost-reversible Type II sorption isotherms. The swelling studies revealed that the presence of hydrogen bonds between the hydroxyl groups of CA with the carbonyl ester groups of PLA limited the ability of CA molecules to leach from the polymer matrix. Water vapor permeability were all determined to be within the range of 15-19 g/m/h. In-vitro cell viability and cell proliferation studies revealed the superiority of the fabricated dressing mats in terms of its bioactivity and cellular interaction. In-vivo studies confirmed the major improvement in its wound healing capabilities attributed to an enhanced epithelization, anti-inflammation, neo-angiogenesis, fibroplasias and collagen deposition that surpassed that of commercially available ones.