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Evaluating the impact of media and feed combinations on CHO cell culture performance and monoclonal antibody (trastuzumab) production.
Cytotechnology
February 2025
Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 USA.
Unlabelled: The choice of media and feeds significantly influences the performance of Chinese Hamster Ovary (CHO) mammalian cell cultures in producing desired biologics like monoclonal antibodies (mAb). Sub-optimal nutrient feed/media composition can severely impact cell proliferation and the quality of the final mAb product. For instance, proper protein glycosylation, crucial for mAb stability, safety, and efficacy, heavily relies on cell culture conditions.
View Article and Find Full Text PDFTranscriptomic and Metabolomic Insights Into the Prebiotic Potential of Camellia Seed Oil for Enhancing Proliferation In Vitro.
Food Sci Nutr
January 2025
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering Guizhou University Guiyang China.
Camellia seed oil (CSO), a potential prebiotic agent, can significantly increase the relative abundance of () in mice gut microbiota following oral administration, this study aims to investigate the enhancing effect in vitro. The results showed that after 24-h co-cultivation with 0.5% (v/v) CSO, the growth of increased from 11.
View Article and Find Full Text PDFThe Impact of Social Media on Disordered Eating: Insights from Israel.
Nutrients
January 2025
Department of Public Health, Ashkelon Academic College, Ashkelon 78211, Israel.
Background: Social media platforms have become integral to daily life and increasingly disseminate health, nutrition, and food information. While these platforms can offer evidence-based nutrition education and meal planning guidance, a significant portion of content promotes unrealistic beauty standards and unhealthy weight-loss practices, potentially contributing to disordered eating behaviors. The increasing prevalence of disordered eating, characterized by abnormal eating behaviors and attitudes, has become a global public health concern.
View Article and Find Full Text PDFNutrition Knowledge and Perceived Dietary Requirements of Adolescent Student-Athletes: A Pilot Study.
Nutrients
December 2024
Patriot Performance Laboratory, Frank Pettrone Center for Sports Performance, Intercollegiate Athletics, George Mason University, Fairfax, VA 22030, USA.
Background/objectives: Adherence to sports-specific nutritional guidelines can help optimize athlete performance and health. However, adolescent athletes may not have adequate nutrition knowledge and understanding of specific nutritional requirements. The objective of the current study was to examine the nutrition knowledge and perceived dietary requirements of adolescent athletes.
View Article and Find Full Text PDFCommon Questions and Misconceptions About Energy Drinks: What Does the Scientific Evidence Really Show?
Nutrients
December 2024
College of Health Professions and Sciences, University of Central Florida, Orlando, FL 32816, USA.
Energy drinks are a commonly consumed beverage, and studies suggest a possible performance-enhancing effect. A Google Scholar search using the keywords "energy drinks" and "exercise" yields numerous results, underscoring the voluminous research on this topic. However, there are questions regarding the effectiveness and safety of energy drinks.
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