Rationale: The influence of hydrophilic additives glycine, glucose, and glycerol on electrospray ionization (ESI) signal intensity of flavonoid glycosides and a nonreducing disaccharide is examined. The addition of excess glycine to the ESI solution would affect signal intensity more than glucose and glycerol due to its strong hydration capability.
Methods: The ESI signal response upon the addition of excess additives prepared was estimated in both selected ion monitoring and scan mode. All the mass spectrometry data were acquired in negative ion mode, because negative ion mode is recommended for saccharide compounds.
Results: The addition of glycine to the ESI solution of flavonoid glycosides and trehalose enhanced signal intensity, whereas the addition of glucose and glycerol had little effect. The signal intensity of rutin was higher than that of naringin and hesperidin, in accordance with their solubility in ESI solution. Trehalose molecules specifically interacted with glycine molecules to form a 1:1 trehalose-glycine complex, whereas the flavonoid glycosides did not produce such complex ions.
Conclusions: The ESI signal enhancement of the saccharides with the additive glycine can be explained by its strong hydration capability, with the deprotonated carboxylic oxygens of zwitterionic glycine molecules strongly interacting with water hydrogen atoms resulting in strong hydration enthalpy. Consequently, glycine molecules set the analytes free from solvation with water molecules in the ESI droplets.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/rcm.8914 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multimodal Pain Recognition in Postoperative Patients: Machine Learning Approach.
JMIR Form Res
January 2025
Department of Computer Science, University of California, Irvine, Irvine, CA, United States.
Background: Acute pain management is critical in postoperative care, especially in vulnerable patient populations that may be unable to self-report pain levels effectively. Current methods of pain assessment often rely on subjective patient reports or behavioral pain observation tools, which can lead to inconsistencies in pain management. Multimodal pain assessment, integrating physiological and behavioral data, presents an opportunity to create more objective and accurate pain measurement systems.
View Article and Find Full Text PDFMonolithic Multiparameter Terahertz Nano/Microdetector Based on Plasmon Polariton Atomic Cavity.
Adv Mater
January 2025
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510275, China.
Terahertz (THz) signals are crucial for ultrawideband communication and high-resolution radar, demanding miniaturized detectors that can simultaneously measure multiple parameters such as intensity, frequency, polarization, and phase. Traditional detectors fail to meet these needs. To address this, we introduce a plasmon polariton atomic cavity (PPAC) detector based on monolayer graphene, offering a multifunctional, monolithic, and miniaturized solution.
View Article and Find Full Text PDFIdentifying Primary Sites of Spinal Metastases: Expert-Derived Features vs. ResNet50 Model Using Nonenhanced MRI.
J Magn Reson Imaging
January 2025
Department of Radiology, Peking University Third Hospital, Beijing, China.
Background: The spinal column is a frequent site for metastases, affecting over 30% of solid tumor patients. Identifying the primary tumor is essential for guiding clinical decisions but often requires resource-intensive diagnostics.
Purpose: To develop and validate artificial intelligence (AI) models using noncontrast MRI to identify primary sites of spinal metastases, aiming to enhance diagnostic efficiency.
Inflammation is a complex host response to harmful infections or injuries, playing both beneficial and detrimental roles in tissue regeneration. Notably, clinical dentinogenesis associated with caries development occurs within an inflammatory environment. Reparative dentinogenesis is closely linked to intense inflammation, which triggers the recruitment and differentiation of dental pulp stem cells (DPSCs) into the dentin lineage.
View Article and Find Full Text PDFUnlabelled: Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!