AI Article Synopsis
- Chromosomes for karyotyping were developed from short-term cell cultures of embryonic northern leopard frogs at different growth stages.
- The process involved mincing embryos in a trypsin solution, then culturing the fragments with fetal calf serum and Colcemid.
- This new technique proved to be more effective than the traditional squash method, resulting in a higher yield of metaphase spreads for better identification and analysis of chromosomal abnormalities.
Article Abstract
Chromosomes suitable for karyotyping were produced from short-term cultures of cells from embryonic northern leopard frogs, Rana pipiens, at various growth stages. Embryos were minced in a trypsin solution. The tissue fragments were subsequently cultured in an electrolytic solution containing fetal calf serum and Colcemid. We found this technique to be highly reproducible. In contrast to the conventional squash technique we were able to obtain a greater number of metaphase spreads suitable for identification and analysis of individual chromosomal aberrations.
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| http://dx.doi.org/10.3109/10520297709116755 | DOI Listing |
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