Aflatoxins and ochratoxin A occur frequently in grains and are associated with carcinogenic, and nephrotoxic properties. Therefore, the aim of this study was to determine the level of aflatoxins and ochratoxin A contamination in wheat samples obtained from different Egyptian governorates and to assess the effect of gamma irradiation on AFB in spiked wheat samples, as well as to evaluate the estimated daily intake and hazard index. Thirty-six wheat grain samples purchased from different sale points were analyzed by High-Performance Liquid Chromatography. Data revealed that 33.33 % of the wheat grain samples were contaminated by aflatoxin B, whereas only 16.66 % of the wheat samples were above the maximum limits (2 μg/kg) set by the European Commission. Ochratoxin A was only detected in two wheat grain samples, and the results were considered below the maximum limit (5 μg/kg) set by the European Commission. On studying the effect of gamma irradiation on wheat samples spiked by aflatoxin B (20 μg/kg), results revealed that aflatoxin B was reduced to 1.22 and 0.94 μg/kg for samples gamma-irradiated at a dose of 10 and 20 KGy respectively. Estimated daily intake of ochratoxin A in wheat samples was found to be higher than that of the tolerable daily intake; however, hazard index values were below one, thus demonstrating no threat to human health.
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| http://dx.doi.org/10.1016/j.toxrep.2020.07.003 | DOI Listing |
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