AI Article Synopsis
Article Abstract
Aims: Brazil is nowadays one of the epicentres of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic and new therapies are needed to face it. In the context of specific immune response against the virus, a correlation between Major Histocompatibility Complex Class I (MHC-I) and the severity of the disease in patients with COVID-19 has been suggested. Aiming at better understanding the biology of the infection and the immune response against the virus in the Brazilian population, we analysed SARS-CoV-2 protein S peptides in order to identify epitopes able to elicit an immune response mediated by the most frequent MHC-I alleles using in silico methods.
Methods: Our analyses consisted in searching for the most frequent Human Leukocyte Antigen (HLA)-A, HLA-B and HLA-C alleles in the Brazilian population, excluding the genetic isolates; then, we performed: molecular modelling for unsolved structures, MHC-I binding affinity and antigenicity prediction, peptide docking and molecular dynamics of the best fitted MHC-I/protein S complexes.
Results: We identified 24 immunogenic epitopes in the SARS-CoV-2 protein S that could interact with 17 different MHC-I alleles (namely, HLA-A*01:01; HLA-A*02:01; HLA-A*11:01; HLA-A*24:02; HLA-A*68:01; HLA-A*23:01; HLA-A*26:01; HLA-A*30:02; HLA-A*31:01; HLA-B*07:02; HLA-B*51:01; HLA-B*35:01; HLA-B*44:02; HLA-B*35:03; HLA-C*05:01; HLA-C*07:01 and HLA-C*15:02) in the Brazilian population.
Conclusions: Being aware of the intrinsic limitations of in silico analysis (mainly the differences between the real and the Protein Data Bank (PDB) structure; and accuracy of the methods for simulate proteasome cleavage), we identified 24 epitopes able to interact with 17 MHC-I more frequent alleles in the Brazilian population that could be useful for the development of strategic methods for vaccines against SARS-CoV-2.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1136/jclinpath-2020-206946 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gut colonization of semi-aquatic turtles inhabiting the Brazilian Amazon by international clones of CTX-M-8-producing Escherichia coli.
Vet Microbiol
December 2024
One Health Brazilian Resistance Project (OneBR), Brazil; Department of Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Electronic address:
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli poses a significant threat to public health due to its ability to confer resistance to broad-spectrum antimicrobials, especially third-generation cephalosporins. Herein, we report gut colonization by international clones of CTX-M-8-producing E. coli in scorpion mud turtles (Kinosternon scorpioides) from a captive breeding program in the Brazilian Amazon.
View Article and Find Full Text PDFBuilding global collaborative research networks in paediatric critical care: a roadmap.
Lancet Child Adolesc Health
December 2024
Department of Pediatrics, University of Ottawa and Children's Hospital of Eastern Ontario and Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada.
Paediatric critical care units are designed for children at a vulnerable stage of development, yet the evidence base for practice and policy in paediatric critical care remains scarce. In this Health Policy, we present a roadmap providing strategic guidance for international paediatric critical care trials. We convened a multidisciplinary group of 32 paediatric critical care experts from six continents representing paediatric critical care research networks and groups.
View Article and Find Full Text PDFDevelopment and cultural adaptation of Text Messages for promoting healthy eating among Amazonian schoolchildren.
Appetite
December 2024
Department of Nutrition, School of Public Health, University of São Paulo, Brazil; Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisboa, Portugal. Electronic address:
Objective: To develop text messages for a mHealth intervention promoting healthy eating among Amazonian schoolchildren.
Design: Two rounds of expert panel analysis and formative research interviews.
Setting: Western Brazilian Amazon, Cruzeiro do Sul, Acre, Brazil.
Impact of health conditions on non-medical costs: a time series analysis of disability insurance and hospital medical costs in Brazil (2010-2019).
BMC Public Health
December 2024
Medical Clinic Department, Federal University of Paraná (UFPR), Curitiba, Brazil.
Background: Disability insurance represents a significant economic burden within Brazil's social security system, yet long-term cost trends across disease groups remain understudied, hindering informed prevention and management strategies. Hospital costs, which account for approximately 40% of direct healthcare expenses, were selected as a comparative reference to contextualize the economic burden of disability insurance.
Objective: This study analyzes long-term cost trends of newly granted disability insurance by disease groups in Brazil, comparing them to public health system hospitalization expenses.
A population study of Toxoplasma gondii in the Amazon region expands current knowledge of the genetic diversity in South America.
PLoS Negl Trop Dis
December 2024
Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America.
Previous studies have reported high diversity between and within populations of Toxoplasma gondii in South America. In the present study, isolates of T. gondii from chickens were obtained from the Amazon region.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!