AI Article Synopsis

  • Silk fibroin nanoparticles are hydrophobic, allowing for effective uptake by the reticulo-endothelial system and macrophages, which is essential for drug delivery.
  • These nanoparticles were modified with Tween-80 to improve circulation time and enable crossing of the blood-brain barrier, making them particularly useful for delivering anti-cancer drugs like doxorubicin.
  • Characterization studies showed high entrapment efficiency and low toxicity, with a sustained drug release pattern that enhanced cytotoxic effects against glioblastoma cells, indicating their potential as an efficient drug delivery system.

Article Abstract

Silk fibroin nanoparticles possess the hydrophobic nature which assists them to become a good substrate for reticulo-endothelial system (RES) and macrophageal uptake. Surface coating of these nanoparticles with hydrophilic stabilizers, like Tween-80 make them long circulating and facilitate their uptake by low density lipoprotein (LDL) receptors to cross blood brain barrier (BBB). Surface modified silk fibroin nanoparticles bearing anti-cancer agent doxorubicin (DOX) were fabricated by desolvation method and coated with Tween-80 as surface modifier. The prepared nanoparticles were characterized for various physicochemical parameters, like particle size, surface charge, surface morphology by scanning electron microscope (SEM) and transmission electron microscopy (TEM), and in vitro drug release along with in vitro cell cytotoxicity, flow cytometry and cellular uptake studies by flourocytometry on glioblastoma cell lines. Entrapment efficiency for the silk fibroin nanoparticles were found to be >85% for coated and uncoated nanoparticles. Nanoparticles with average diameter less than 150 nm having negative charge were found to show no toxicity of its own. The pro-inflammatory response of nanoparticles was observed by determining the cytokines level, such as TNF-α and IL-1β. Sustained drug release pattern from the nanoparticles with better cytotoxicty as compared to free drug was observed, signifying their potential ability to work as a drug delivery system.

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.326DOI Listing

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