AI Article Synopsis
- There is a growing need for new antibiotics due to antibiotic-resistant bacteria like MRSA, and researchers discovered a new strain, MUSC 125, from mangrove soil in Malaysia that shows promise against this issue.
- The methanolic extract from strain MUSC 125 exhibited anti-MRSA, anti-biofilm, and antioxidant properties, and contained specific polyketide synthase gene clusters suggested to be linked to these effects.
- Chemical analysis identified eight compounds in the extract that could contribute to its antibiotic activity, indicating that strain MUSC 125 may be a valuable resource for developing new anti-MRSA treatments.
Article Abstract
There is an urgent need to search for new antibiotics to counter the growing number of antibiotic-resistant bacterial strains, one of which is methicillin-resistant (MRSA). Herein, we report a sp. strain MUSC 125 from mangrove soil in Malaysia which was identified using 16S rRNA phylogenetic and phenotypic analysis. The methanolic extract of strain MUSC 125 showed anti-MRSA, anti-biofilm and antioxidant activities. Strain MUSC 125 was further screened for the presence of secondary metabolite biosynthetic genes. Our results indicated that both polyketide synthase () gene clusters, I and II, were detected in strain MUSC 125 by PCR amplification. In addition, gas chromatography-mass spectroscopy (GC-MS) detected the presence of different chemicals in the methanolic extract. Based on the GC-MS analysis, eight known compounds were detected suggesting their contribution towards the anti-MRSA and anti-biofilm activities observed. Overall, the study bolsters the potential of strain MUSC 125 as a promising source of anti-MRSA and antibiofilm compounds and warrants further investigation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435833 | PMC |
| http://dx.doi.org/10.3390/molecules25153545 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adaptations in glutathione-based redox protein signaling pathways and alcohol drinking across species.
Biomed Pharmacother
November 2024
Department of Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA. Electronic address:
Alcohol use disorder (AUD) is the most prevalent substance use disorder but there is incomplete knowledge of the underlying molecular etiology. Here, we examined the cytosolic proteome from the nucleus accumbens core (NAcC) of ethanol drinking rhesus macaques to identify ethanol-sensitive signaling proteins. The targets were subsequently investigated using bioinformatics, genetic, and pharmacological manipulations in mouse models of ethanol drinking.
View Article and Find Full Text PDFTransgenic expression of human cytochrome P450 2E1 in C. elegans and rat PC-12 cells sensitizes to ethanol-induced locomotor and mitochondrial effects.
Biochem Biophys Res Commun
November 2024
Dept of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Ave. BSB 501 | MSC 509, Charleston, SC, 29425, USA; Dept of Regenerative Medicine and Cell Biology, Medical University of South Carolina, 173 Ashley Ave. BSB 501 | MSC 509, Charleston, SC, 29425, USA. Electronic address:
Chronic alcohol (ethanol) use is increasing in the United States and has been linked to numerous health issues in multiple organ systems including neurological dysfunction and diseases. Ethanol toxicity is mainly driven by the metabolite acetaldehyde, which is generated through three pathways: alcohol dehydrogenase (ADH2), catalase (CAT), and cytochrome P450 2E1 (CYP2E1). ADH2, while the main ethanol clearance pathway in the liver, is not expressed in the mammalian brain, resulting in CAT and CYP2E1 driving local metabolism of ethanol in the central nervous system.
View Article and Find Full Text PDFExplainable deep learning and biomechanical modeling for TMJ disorder morphological risk factors.
JCI Insight
July 2024
Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering and.
Clarifying multifactorial musculoskeletal disorder etiologies supports risk analysis, development of targeted prevention, and treatment modalities. Deep learning enables comprehensive risk factor identification through systematic analyses of disease data sets but does not provide sufficient context for mechanistic understanding, limiting clinical applicability for etiological investigations. Conversely, multiscale biomechanical modeling can evaluate mechanistic etiology within the relevant biomechanical and physiological context.
View Article and Find Full Text PDFA natural loss-of-function deletion of the cytohesin 1 (Cyth1) gene in BALB/cByJ mice does not impact cardiomyocyte polyploidy.
Sci Rep
June 2024
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, USA.
Mammalian cardiomyocytes (CMs) mostly become polyploid shortly after birth. Because this feature may relate to several aspects of heart biology, including regeneration after injury, the mechanisms that cause polyploidy are of interest. BALB/cJ and BALB/cByJ mice are highly related sister strains that diverge substantially in CM ploidy.
View Article and Find Full Text PDFBackground: There are currently no proven methods to reverse muscle loss in humans, which is caused by trauma (e.g., volumetric muscle loss, VML), genetic neuromuscular diseases (e.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!