Background: Premature rupture of membrane (PROM) remains a problem in obstetrics, the mechanisms of PROM have not been clearly defined. Apoptosis is thought to play a key role in the mechanism, via caspase-dependent and caspase-independent pathways. Caspase-3, Apoptosis-inducing factor (AIF), and anti-apoptosis B-cell lymphoma 2 (Bcl-2) are hypothesized to be involved in PROM.
Objective: To determine the role of caspase-dependent and caspase-independent pathways in the mechanism of PROM.
Materials And Methods: This was a case-control study involving 42 pregnant women with gestational age between 20-42 wk. Participants were divided into the case group (with PROM) and control group (without PROM). Amniotic membranes were collected immediately after the delivery, and samples were taken from the site of membrane rupture. Immunohistochemical examination was done to determine the expression of Caspase-3, AIF, and Bcl-2.
Results: The expressions of Caspase-3 (OR = 9.75; 95% CI = 2.16-43.95; p = 0.001) and AIF (OR = 6.60; 95% CI = 1.48-29.36; p = 0.009) were significantly increased, whereas, Bcl-2 expressions (OR = 8.00; 95% CI = 1.79-35.74; p = 0.004) were significantly decreased in the case group.
Conclusion: High Caspase-3, AIF, and low Bcl-2 expression were the risk factors for PROM. Thus, it is evident that caspase-dependent and caspase-independent pathways are involved in the mechanism of PROM.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340985 | PMC |
| http://dx.doi.org/10.18502/ijrm.v13i6.7285 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unveiling the susceptibility of nanosecond pulsed electric field on intracellular function in breast cancerous and normal cells using fluorescence imaging.
Biosens Bioelectron
March 2025
Department of Applied Chemistry and Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan; Center for Emergent Functional Matter Science, National Yang Ming Chiao Tung University, 1001 Ta-Hsueh Rd., Hsinchu, 300093, Taiwan. Electronic address:
Modulation in cellular function and cell death through electrostimulation of intracellular organelles with the application of 50 ns pulsed electric field (nsPEF) have been investigated in breast cancerous MCF7 and normal MCF10A cells by developing a three-dimensional microelectrode device integrated with a fluorescence microscope. The findings revealed that nsPEF induced distinct effects on intracellular functions and dynamics in MCF7 and MCF10A cells. MCF10A cells exhibited significantly higher survivability than MCF7 cells, with different modes of cell death observed between them.
View Article and Find Full Text PDFFucoidan from Induces Apoptosis through Caspase Dependent and Caspase-Independent Activation in 4T1 Breast Adenocarcinoma In Vitro.
Mar Drugs
May 2024
Research Group Immunomodulators and Antitumor of Natural and Synthetic Origen, Immunology Laboratory, Universidad Nacional Mayor de San Marcos, Lima 11-0058, Peru.
Experiments conducted on triple-negative breast cancer have shown that fucoidan from (FLt) exhibits cytotoxic and antitumor properties. However, further research is necessary to gain a complete understanding of its bioactivity and level of cytotoxicity. The cytotoxic effect of FLt was determined by the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
View Article and Find Full Text PDFHost Cell Death and Modulation of Immune Response against Infection.
Int J Mol Sci
June 2024
College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA.
() is the causative agent of tuberculosis (TB), a prevalent infectious disease affecting populations worldwide. A classic trait of TB pathology is the formation of granulomas, which wall off the pathogen, via the innate and adaptive immune systems. Some key players involved include tumor necrosis factor-alpha (TNF-α), foamy macrophages, type I interferons (IFNs), and reactive oxygen species, which may also show overlap with cell death pathways.
View Article and Find Full Text PDFUnraveling the intriguing interplay: Exploring the role of lncRNAs in caspase-independent cell death.
Wiley Interdiscip Rev RNA
June 2024
Noncoding RNA Laboratory, Department of Molecular Biology and Genetics, Izmir Institute of Technology, Izmir, Gülbahçeköyü, Urla, Turkey.
Cell death plays a crucial role in various physiological and pathological processes. Until recently, programmed cell death was mainly attributed to caspase-dependent apoptosis. However, emerging evidence suggests that caspase-independent cell death (CICD) mechanisms also contribute significantly to cellular demise.
View Article and Find Full Text PDFThe mechanism of reactive oxygen species generation, DNA damage and apoptosis in hemocytes of Litopenaeus vannamei under ammonia nitrogen exposure.
Aquat Toxicol
July 2024
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China. Electronic address:
Ammonia-N poses a significant threat to aquatic animals. However, the mechanism of ROS production leading to DNA damage in hemocytes of crustaceans is still unclear. Additionally, the mechanism that cells respond to DNA damage by activating complex signaling networks has not been well studied.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!