A technique has been devised which optimally demonstrates non-specific esterase activity in human blood lymphocytes. The reaction is carried out on smears fixed with formalin vapour, using alpha-naphthol butyrate as substrate at pH 8 and at a low concentration for a short incubation period. The pattern of esterase activity revealed by this method provides a discriminating marker for mature T-lymphocytes, which show dense, localised, dot-like positivity, and a probable marker for 'null' cells in the form of scattered granular positivity. B cells appear to be negative. Monocytes show a clearly different pattern of granular positivity.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1111/j.1600-0609.1977.tb02098.x | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!