AI Article Synopsis
- * Although there were no significant differences in overall seroprevalence between the two tests, they showed poor agreement, indicating potential inconsistencies in their results.
- * The IDEXX ELISA with a moderate cutoff value demonstrated better sensitivity (38.1%) than the NVSL ELISA (19.1%), but both tests had varying sensitivity and specificity, with optimal cutoff values indicating how well each test performed.
Article Abstract
Control of infection in sheep flocks in the United States depends on early detection of antibodies via serologic testing. We used 2,276 sheep sera and various cutoff values to compare seroprevalence and agreement between 2 ELISAs: the National Veterinary Services Laboratories (NVSL) indirect ELISA and the IDEXX ELISA kit. A subset of 295 sera was used to compare agreement and evaluate relative sensitivity and specificity of the 2 ELISAs with an agar gel immunodiffusion (AGID) test kit. There was no significant difference in seroprevalence between the ELISAs; however, there was poor agreement between them. When the AGID test was used as the reference test, the IDEXX ELISA with a moderate cutoff value (S/P ratio = 45%) had the highest relative sensitivity of 38.1% and specificity of 92.0%. The NVSL ELISA with a lax cutoff value (S/P ratio = 0.75) had relative sensitivity of 19.1% and specificity of 94.6%. Receiver operating characteristic analysis revealed that optimal cutoff values for the NVSL and IDEXX ELISAs were 0.091 and 16.5%, respectively. This results in sensitivity and specificity of 85.7% and 31.8% for the NVSL ELISA, and sensitivity and specificity of 81.0% and 53.6% for the IDEXX ELISA, respectively.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488971 | PMC |
| http://dx.doi.org/10.1177/1040638720943880 | DOI Listing |
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