AI Article Synopsis
- The study focuses on isolating and studying a specific lectin from the haemolymph of the Penaeus semisulcatus prawn, highlighting its purification and characterization.
- The purified lectin shows a single protein band at 66 kDa, confirmed through techniques like SDS-PAGE, HPLC, and X-ray diffraction.
- Additionally, the lectin demonstrates activities such as agglutination of yeast and human red blood cells, along with antibacterial properties against certain harmful bacteria, indicating its potential use in aquaculture for managing diseases.
Article Abstract
Penaeid prawns are considered as most demanding fishery resources. The current study aims to purify and characterize lectin from the haemolymph of Penaeus semisulcatus. The semisulcatus-lectin was purified by affinity chromatography using mannose coupled Sepharose CL-4B column and purified lectin exhibited a single band of 66 kDa in SDS-PAGE. The purity and crystalline structure of purified lectin was confirmed by HPLC and X-ray diffraction analysis. Semisulcatus-lectin exhibited yeast agglutination activity against Saccharomyces cerevisiae and agglutinated human erythrocytes. Semisulcatus-lectin was evaluated for phenol oxidase activation and phagocytic activities. It was observed that semisulcatus-lectin had antibacterial activity against Gram-negative Vibrio parahaemolyticus and Aeromonas hydrophila, suggesting a potential therapeutic strategy in aquaculture industry for disease management.
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| http://dx.doi.org/10.1016/j.fsi.2020.07.053 | DOI Listing |
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