AI Article Synopsis
- Immotile cilia in mouse embryos help sense fluid flow and play a role in breaking left-right (L-R) symmetry through calcium (Ca) signaling.
- Intraciliary and cytoplasmic Ca transients were identified in crown cells, showing that these signals are asymmetrically biased and rely on fluid flow and the PKD2 channel.
- The study categorized the Ca transients into two types, revealing that type 1 (L-R asymmetric) signals are crucial for initiating L-R symmetry breaking, particularly in the left posterior region of the node.
Article Abstract
Immotile cilia sense extracellular signals such as fluid flow, but whether Ca plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca transients were detected in the crown cells at the node. These Ca transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375832 | PMC |
| http://dx.doi.org/10.1126/sciadv.aba1195 | DOI Listing |
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