AI Article Synopsis
- The study investigates how an external electric field affects the behavior of single-walled carbon nanotubes (SWCNTs) in glioma cells, focusing on their accumulation, distribution, and clumping.
- C6 glioma cells were treated with SWCNTs and exposed to specific electric pulses, finding that the electric field enhances the uptake of nanotubes by the cells.
- Results showed a significant increase in the number of nanotubes within cell agglomerates after electric field stimulation, suggesting that this method could improve the effectiveness of delivering anticancer drugs while reducing the required doses.
Article Abstract
The objective of the study is to determine the patterns of regulation of single-walled carbon nanotube accumulation, distribution, and agglomeration in glioma cells exposed to an external electric field. C6 glioma cells were treated with 5 μg/ml DNA wrapped single-walled carbon nanotubes and exposed to bi-phasic electric pulses (6.6 V/m, 200 Hz, pulse duration 1 ms). Nanotube accumulation was determined by Raman microspectroscopy and their intracellular local concentration was evaluated using the G-band intensity in Raman spectra of single-walled carbon nanotubes. It was revealed that the low-frequency and low-strength electric field stimulation of glioma cells exposed to single-walled carbon nanotubes led to facilitation and, thus, to amplification of nanotube accumulation inside the cells. The number of nanotubes in intracellular agglomerates increased from (28.8 ± 13.1) un./agglom. and (84.0 ± 28.7) un./agglom. in control samples to (60.6 ± 21.4) un./agglom. and (184.2 ± 53.4) un./agglom. for 1 h and 2 h stimulation, respectively. Thus, the tumor exposure to an external electric field makes it possible to more effectively regulate the accumulation and distribution of carbon nanotubes inside glioma cells allowing to reduce the applied therapeutic doses of carbon nanomaterial delivered anticancer drugs.
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| http://dx.doi.org/10.1016/j.bbrc.2020.06.100 | DOI Listing |
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