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Effect of endothelial cell heterogeneity on nanoparticle uptake. | LitMetric

Effect of endothelial cell heterogeneity on nanoparticle uptake.

Int J Pharm

Department of Nanomedicine & Drug Targeting, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands. Electronic address:

Published: September 2020

AI Article Synopsis

  • - Endothelial cells from various organs (brain, lung, liver, kidney) show unique characteristics that can be utilized for targeted nanomedicine, as they react differently to modified silica nanoparticles.
  • - The study found that the protein coronas from different nanoparticle types and serum (FBS vs. human serum) influenced how effectively these cells incorporated nanoparticles; specifically, uptake patterns varied depending on the type of silica used.
  • - Differences in nanoparticle uptake efficiency among organ-specific endothelial cells are likely due to variations in receptor expression and activity, highlighting the importance of understanding these phenotypic differences for improving nanomedicine applications.

Article Abstract

Endothelial cells exhibit distinct properties in morphology and functions in different organs that can be exploited for nanomedicine targeting. In this work, endothelial cells from different organs, i.e. brain, lung, liver, and kidney, were exposed to plain, carboxylated, and amino-modified silica. As expected, different protein coronas were formed on the different nanoparticle types and these changed when foetal bovine serum (FBS) or human serum were used. Uptake efficiencies differed strongly in the different endothelia, confirming that the cells retained some of their organ-specific differences. However, all endothelia showed higher uptake for the amino-modified silica in FBS, but, interestingly, this changed to the carboxylated silica when human serum was used, confirming that differences in the protein corona affect uptake preferences by cells. Thus, uptake rates of fluid phase markers and transferrin were determined in liver and brain endothelium to compare their endocytic activity. Overall, our results showed that endothelial cells of different organs have very different nanoparticle uptake efficiency, likely due to differences in receptor expression, affinity, and activity. A thorough characterization of phenotypic differences in the endothelia lining different organs is key to the development of targeted nanomedicine.

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Source
http://dx.doi.org/10.1016/j.ijpharm.2020.119699DOI Listing

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