In this work, a quartz crystal microbalance (QCM) sensor has been fabricated using immunoassay for sensitive determination of . Au nanoparticle has been used for amplifying sandwich assays. The proposed immunosensor exhibited a linear detection range between 10 and 10 CFU/mL with a limit of detection of 2.1 × 10 CFU/mL. The proposed immunosensor exhibited good selectivity for sensing with low cross reactivity for other foodborne pathogens such as , and . In addition, the proposed immunosensor has been successfully used for detection in feces samples and food samples. The frequency decreases of 12, 17, and 10 Hz were observed from the milk samples consisting of the mixtures of , and . The frequency decreases of 8, 15, and 7 Hz were observed from the feces samples consisting of the mixtures of , and .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358898PMC
http://dx.doi.org/10.3389/fchem.2020.00548DOI Listing

Publication Analysis

Top Keywords

proposed immunosensor
12
detection feces
8
quartz crystal
8
crystal microbalance
8
immunosensor exhibited
8
feces samples
8
frequency decreases
8
decreases observed
8
samples consisting
8
consisting mixtures
8

Similar Publications

Alzheimer's disease (AD) is characterized by progressive memory loss and cognitive decline, significantly impairing the daily life of elderly individuals. The low abundance of blood-based biomarkers in AD necessitates higher analytical technique requirements. Herein, one novel iridium-based ECL self-enhanced nanoemitter (TPrA@Ir-SiO) was unprecedentedly reported, and it was further used to construct an ultrasensitive ECL magnetic immunosensor by a multiple-signal amplification strategy to unequally sensitively and accurately detect the AD blood-based biomarker (P-tau181) in this work.

View Article and Find Full Text PDF

Target-regulated AgS/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor.

Mikrochim Acta

January 2025

College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Zhejiang Normal University, Jinhua, 321004, China.

Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of AgS/FeOOH heterojunction. The AgS/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb.

View Article and Find Full Text PDF

Potential-resolved electrochemiluminescent immunoassay based on dual co-reactants regulation.

Biosens Bioelectron

December 2024

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. Electronic address:

Multi-signal-based self-calibrating biosensors have become a research focus due to their superior accuracy and sensitivity in recent years. Herein, the potential-resolved differential ECL immunoassay based on dual co-reactants regulation was developed. Meso-tetra(4-carboxyphenyl)porphyrin (TCPP) functionalized zirconium dioxide (ZrO) composites (TCPP-ZrO) was first synthesized using TCPP as the luminophore and ZrO as the enhancer and stabilizer.

View Article and Find Full Text PDF

A highly sensitive creatine kinase detection in human serum using 11-mercaptoundecanoic acid modified ITO-PET electrodes.

Anal Biochem

January 2025

Çanakkale Onsekiz Mart University, Faculty of Engineering, Bioengineering Department, Çanakkale, Turkey. Electronic address:

The enzyme creatine kinase (CK) is a biomarker that plays an extremely significant role in the early detection of cardiovascular disorders. Serum levels of CK are regularly monitored in patients with heart attacks, one of the most critical cardiovascular illnesses. In this study, a highly sensitive electrochemical immunosensor system was designed for the importance of early diagnosis of CK.

View Article and Find Full Text PDF

This study developed an electrochemical immunosensor for the detection of aflatoxin B1 (AFB1) in vegetable oil, based on an electrochemical modified carbon cloth (EMCC) electrode modified with a composite functional layer of cross-linked o-aminothiophenol functionalized AuNPs (o-ATP@AuNPs)/Prussian Blue (PB). The EMCC electrode substrate was prepared by modifying carbon cloth through electrochemical methods to increase its surface area, which allowed for the effective deposition of o-ATP@AuNPs/PB composite functional layer and improved the conductivity of the electrode material. The synergistic effect of o-ATP@AuNPs and PB significantly enhanced the sensitivity of the electrochemical sensor.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!